Cruz Nelly M, Freedman Benjamin S
Department of Medicine, Division of Nephrology, University of Washington School of Medicine, Seattle, WA, United States; Kidney Research Institute, University of Washington School of Medicine, Seattle, WA, United States; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA, United States; Department of Pathology, University of Washington School of Medicine, Seattle, WA, United States.
Department of Medicine, Division of Nephrology, University of Washington School of Medicine, Seattle, WA, United States; Kidney Research Institute, University of Washington School of Medicine, Seattle, WA, United States; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA, United States; Department of Pathology, University of Washington School of Medicine, Seattle, WA, United States.
Methods Cell Biol. 2019;153:133-150. doi: 10.1016/bs.mcb.2019.05.003. Epub 2019 Jun 19.
It is now possible to direct the differentiation of human pluripotent stem cells into three-dimensional nephron-like structures called kidney organoids. Organoids contain proximal and distal tubules as well as podocytes, in addition to a variety of other lineages such as endothelial cells. Organoid technology has great potential for kidney regeneration and has already been proven to be suitable for modeling kidney disease. However, the methodologies that are used for the generation of kidney organoids require expertise and can be daunting for the inexperienced. Here, we describe in detail a well-established and relatively simple method for the generation of human kidney organoids. We include notes on technical and design considerations for these experiments, and highlight key advantages and limitations of the system.
现在已经能够将人类多能干细胞分化为称为肾类器官的三维肾单位样结构。类器官除了含有多种其他细胞谱系（如内皮细胞）外，还包含近端和远端肾小管以及足细胞。类器官技术在肾脏再生方面具有巨大潜力，并且已经被证明适用于模拟肾脏疾病。然而，用于生成肾类器官的方法需要专业知识，对于没有经验的人来说可能具有挑战性。在这里，我们详细描述一种成熟且相对简单的生成人类肾类器官的方法。我们包括这些实验的技术和设计考虑要点，并突出该系统的主要优点和局限性。
