Chen Lulu, Wang Guantong, Wang Qinjue, Liu Quan, Sun Qiang, Chen Lulu
Department of Anatomy, Histology and Embryology, Nanjing Medical University Nanjing 211166, Jiangsu, China.
Key Laboratory for Aging and Disease, Histology and Embryology, Nanjing Medical University Nanjing 211166, Jiangsu, China.
Am J Transl Res. 2019 Jul 15;11(7):4337-4347. eCollection 2019.
Oxidative stress is associated with many diseases and has been found to induce DNA damage and cellular senescence. Numerous evidences support the detrimental effects of oxidative stress or cellular senescence on skeletal homeostasis. N-acetylcysteine (NAC) is a powerful antioxidant. However, it is unclear whether NAC can suppress orchiectomy (ORX)-induced osteoporosis by inhibiting oxidative stress and osteocyte senescence. In this study, ORX mice were supplemented with/without NAC, and were compared with each other and with sham-operated mice. Our results showed that NAC could prevent ORX-induced osteoporosis by inhibiting oxidative stress, DNA damage, osteocyte senescence and senescence-associated secretory phenotype (SASP), subsequently stimulating osteoblastic bone formation and inhibiting osteoclastic bone resorption. The results from this study suggest that NAC could be considered as a potential therapeutic agent for prevention and treatment of osteoporosis caused by testosterone deficiency.
氧化应激与多种疾病相关，并且已发现其可诱导DNA损伤和细胞衰老。大量证据支持氧化应激或细胞衰老对骨骼稳态的有害影响。N-乙酰半胱氨酸（NAC）是一种强大的抗氧化剂。然而，目前尚不清楚NAC是否能够通过抑制氧化应激和骨细胞衰老来抑制去势（ORX）诱导的骨质疏松症。在本研究中，对去势小鼠补充或不补充NAC，并将其相互比较，同时与假手术小鼠进行比较。我们的结果表明，NAC可通过抑制氧化应激、DNA损伤、骨细胞衰老和衰老相关分泌表型（SASP），随后刺激成骨细胞骨形成并抑制破骨细胞骨吸收，从而预防去势诱导的骨质疏松症。本研究结果表明，NAC可被视为预防和治疗由睾酮缺乏引起的骨质疏松症的潜在治疗药物。
