Department of Drug Science, Biochemistry Section, University of Catania, Catania, Italy.
Department of Biomedical and Biotechnological Sciences, Biochemistry Section, University of Catania, Catania, Italy.
Nat Prod Res. 2021 Jun;35(12):2076-2081. doi: 10.1080/14786419.2019.1650352. Epub 2019 Aug 9.
L. (common walnut) is a deciduous tree belonging to Juglandaceae family. Since ancient time, walnut was widely used in traditional medicine for its antioxidant, antidiabetic, antimicrobial, anti-inflammatory, anti-atherogenic and liver-protective effects. In this work, the antibacterial and anti-biofilm activities of walnuts pellicle extract against coagulase-negative staphylococci were evaluated. Qualitative chemical analysis was performed by the thin layer chromatography. UPLC-Ms/Ms was used to identify the chemical composition of extract. The total flavonoid and phenolic contents were determined by the Aluminium chloride and Folin-Ciocalteu methods, respectively. The extract showed antibacterial activity with MIC ranging from 3.60 to 461.75 µg/ml and MBC ranging from 461.75 to >461.75 µg/ml. Furthermore, it significantly reduced biofilm biomass and cell viability in a dose-dependent manner. Biological activities of extract may be due to its high flavonoid and phenolic contents. The obtained results are promising and they deserve further scientific investigations.
（普通胡桃）是胡桃科的落叶乔木。自古以来，核桃因其具有抗氧化、抗糖尿病、抗菌、抗炎、抗动脉粥样硬化和保肝作用，被广泛应用于传统医学。在这项工作中，评估了核桃种皮提取物对凝固酶阴性葡萄球菌的抗菌和抗生物膜活性。采用薄层色谱法进行定性化学分析。采用 UPLC-Ms/MS 鉴定提取物的化学成分。采用铝氯化物和 Folin-Ciocalteu 法分别测定总黄酮和总酚的含量。提取物的 MIC 范围为 3.60 至 461.75μg/ml，MBC 范围为 461.75 至>461.75μg/ml，具有抗菌活性。此外，它还能以剂量依赖的方式显著降低生物膜生物量和细胞活力。提取物的生物活性可能与其高含量的类黄酮和酚类物质有关。所得结果很有前景，值得进一步的科学研究。
