Department of Nutrition and Food Hygiene, School of Public Health, Lanzhou University, Lanzhou 730000, People's Republic of China.
State Key Laboratory of Veterinary of Etiological Biology, Key Laboratory of Veterinary Public Health of Agricultural Ministry, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, People's Republic of China.
Br J Nutr. 2019 Nov 14;122(9):986-995. doi: 10.1017/S0007114519001909.
Altered gut microbial ecology contributes to the development of metabolic diseases including obesity. However, studies based on different populations have generated conflicting results due to diet, environment, methodologies, etc. The aim of our study was to explore the association between gut microbiota and BMI in Chinese college students. The 16S next-generation sequencing (NGS) was used to test the gut microbiota of nine lean, nine overweight/obesity and ten normal-weight male college students. The differences in gut microbiota distribution among three groups were compared, and the relationship between the richness, diversity, composition of gut microbiota and BMI were analysed. The predominant phyla Bacteroidetes and Firmicutes were further confirmed by real-time PCR. Metagenomic biomarker discovery was conducted by linear discriminant analysis (LDA) effect size (LEfSe). NGS revealed that gut microbiota composition was different among three groups, but there was no difference in the abundance ratio of Firmicutes:Bacteroidetes. Several bacterial taxa were in linear relationship with BMI (positive relationship: uncultured bacterium (Bacteroides genus); negative relationship: Porphyromonadaceae, Acidaminococcaceae, Rikenellaceae, Desulfovibrionaceae, Blautia, Anaerotruncus, Parabacteroides, Alistipes). Moreover, gut microbiota diversity decreased with the increase in BMI. And LEfSe analysis indicated that Blautia, Anaerotruncus and its uncultured species were significantly enriched in the lean group (LDA score ≥ 3), Parasuterella and its uncultured species were significantly enriched in the overweight/obese groups (LDA score ≥ 3). In general, gut microbiota composition and microbial diversity were associated with BMI in Chinese male college students. Our results might enrich the understanding between gut microbiota and obesity.
肠道微生物生态的改变有助于代谢性疾病（包括肥胖）的发展。然而，由于饮食、环境、方法等因素，基于不同人群的研究产生了相互矛盾的结果。本研究旨在探索中国大学生肠道微生物群与 BMI 之间的关系。使用 16S 下一代测序（NGS）测试了 9 名瘦、9 名超重/肥胖和 10 名正常体重的男性大学生的肠道微生物群。比较了三组肠道微生物群分布的差异，并分析了肠道微生物群丰富度、多样性、组成与 BMI 的关系。通过实时 PCR 进一步证实了主要的菌门厚壁菌门和拟杆菌门。通过线性判别分析（LDA）效应大小（LEfSe）进行宏基因组生物标志物发现。NGS 显示，三组肠道微生物群组成不同，但厚壁菌门：拟杆菌门的丰度比例没有差异。一些细菌类群与 BMI 呈线性关系（正相关：未培养的细菌（拟杆菌属）；负相关：卟啉单胞菌科、酸杆菌科、真杆菌科、脱硫弧菌科、布劳特氏菌属、厌氧真杆菌属、副拟杆菌属、拟杆菌属）。此外，随着 BMI 的增加，肠道微生物多样性降低。LEfSe 分析表明，布劳特氏菌属、厌氧真杆菌属及其未培养物种在瘦组中明显富集（LDA 评分≥3），副拟杆菌属及其未培养物种在超重/肥胖组中明显富集（LDA 评分≥3）。总的来说，中国男性大学生的肠道微生物群组成和微生物多样性与 BMI 相关。我们的结果可能丰富了对肠道微生物群与肥胖之间关系的理解。
