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温度胁迫下甜玉米(L.)幼苗中维生素 E 和类胡萝卜素积累的比较评价。

Comparative Evaluation on Vitamin E and Carotenoid Accumulation in Sweet Corn ( L.) Seedlings under Temperature Stress.

机构信息

Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center) , School of Food Science and Engineering, South China University of Technology , Guangzhou 510640 , China.

Key Laboratory of Crops Genetics Improvement of Guangdong Province , Crop Research Institute, Guangdong Academy of Agricultural Sciences , Guangzhou , 510640 , China.

出版信息

J Agric Food Chem. 2019 Sep 4;67(35):9772-9781. doi: 10.1021/acs.jafc.9b04452. Epub 2019 Aug 22.

Abstract

This study aims to investigate the response profiles of vitamin E and carotenoids on transcription and metabolic levels of sweet corn seedlings under temperature stress. The treated temperatures were set as 10 °C (low temperature, LT), 25 °C (control, CK), and 40 °C (high temperature, HT) for sweet corn seedlings. The gene expression profiles of vitamin E and carotenoids biosynthesis pathways were analyzed by real time quantitative polymerase chain reaction (RT-qPCR), and the composition profiles were analyzed by high performance liquid chromatography (HPLC). Results showed that vitamin E gradually accumulated in response to LT stress but was limited by HT stress. The increase of carotenoids was suppressed by LT stress whereas HT stress promoted it. The existing results elaborated the interactive and competitive relationships of vitamin E and carotenoids in sweet corn seedlings to respond to extreme temperature stress at transcriptional and metabolic levels. The present study would improve sweet corn temperature resilience with integrative knowledge in the future.

摘要

本研究旨在探究维生素 E 和类胡萝卜素在温度胁迫下甜玉米幼苗转录和代谢水平上的响应特征。处理温度设置为 10°C(低温,LT)、25°C(对照,CK)和 40°C(高温,HT)。通过实时定量聚合酶链反应(RT-qPCR)分析维生素 E 和类胡萝卜素生物合成途径的基因表达谱,并通过高效液相色谱法(HPLC)分析组成谱。结果表明,维生素 E 逐渐积累以响应 LT 胁迫,但受到 HT 胁迫的限制。LT 胁迫抑制类胡萝卜素的增加,而 HT 胁迫则促进其增加。现有结果详细阐述了维生素 E 和类胡萝卜素在甜玉米幼苗中相互作用和竞争关系,以在转录和代谢水平上应对极端温度胁迫。本研究将为未来提高甜玉米的温度适应能力提供综合知识。

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