School of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, China.
Crop Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Sci Rep. 2017 Aug 8;7(1):7484. doi: 10.1038/s41598-017-07774-9.
This study investigates the potential interrelationship between gene expression and biosynthesis of vitamin C, E and folate in sweet corn sprouts. Germination of sweet corn kernels was conducted in light and dark environments to determine if this relationship was regulated by photo-illumination. Results indicated that light and dark environments affected the DHAR, TMT and GTPCH expression and that these genes were the predominant genes of vitamin C, E and folate biosynthesis pathways respectively during the germination. Levels of vitamin C and folate increased during the germination of sweet corn seeds while vitamin E had a declining manner. Sweet corn sprouts had higher vitamin C and E levels as well as relevant gene expression levels in light environment while illumination had little influence on the folate contents and the gene expression levels during the germination. These results indicate that there might be a collaborative relationship between vitamin C and folate regulation during sweet corn seed germination, while an inhibitive regulation might exist between vitamin C and E.
本研究调查了甜玉米芽中基因表达与维生素 C、E 和叶酸生物合成之间的潜在相互关系。在光照和黑暗环境中进行甜玉米籽粒的萌发，以确定这种关系是否受光照射调节。结果表明，光照和黑暗环境影响 DHAR、TMT 和 GTPCH 的表达，并且在萌发过程中，这些基因分别是维生素 C、E 和叶酸生物合成途径的主要基因。在甜玉米种子萌发过程中，维生素 C 和叶酸的水平增加，而维生素 E 呈下降趋势。甜玉米芽在光照环境中具有更高的维生素 C 和 E 水平以及相关基因表达水平，而光照对叶酸含量和萌发过程中的基因表达水平几乎没有影响。这些结果表明，在甜玉米种子萌发过程中，维生素 C 和叶酸的调节可能存在协同关系，而维生素 C 和 E 之间可能存在抑制调节关系。
