School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 252-5201, Japan.
Faculty of Bioresources and Environmental Science, Ishikawa Prefectural University, Nonoichi, Ishikawa 921-8836, Japan.
Virus Res. 2019 Oct 2;271:197680. doi: 10.1016/j.virusres.2019.197680. Epub 2019 Aug 6.
Sapoviruses (SaVs) are enteric viruses that have been detected in human and animals previously; however, SaVs have not been identified in wild boar yet. Using a metagenomics approach, we identified SaVs in fecal samples of free-living wild boars in Japan for the first time. Six of the 48 specimens identified belonged to one genogroup (G)III, one GV and four GVI SaV sequence reads. We successfully determined complete genome of GV and GVI SaV strains using the long reverse transcription PCR strategy and the 5' rapid amplification of cDNA end method. Phylogenetic tree analysis and pairwise distance calculation revealed that GV SaV detected from wild boar was related to recently assigned GV.5 strains from pig, while GVI SaV was assigned to a new genotype within GVI. Moreover, wild boar may act as a reservoir for transmission of SaVs to the pig population (and vice versa) because GIII, GV, and GVI SaVs were all detected in pigs previously.
肠道病毒（SaVs）此前已在人类和动物中被检测到，但尚未在野猪中发现 SaVs。本研究采用宏基因组学方法首次在日本自由放养野猪的粪便样本中鉴定出 SaVs。在 48 个鉴定样本中，有 6 个属于一个基因型 (G)III、一个 GV 和四个 GVI SaV 序列。我们成功地使用长反转录 PCR 策略和 5'快速扩增 cDNA 末端方法确定了 GV 和 GVI SaV 株的完整基因组。系统进化树分析和成对距离计算表明，从野猪中检测到的 GV SaV 与最近从猪中分离到的 GV.5 株有关，而 GVI SaV 被归为 GVI 中的一个新基因型。此外，因为此前已在猪中检测到 GIII、GV 和 GVI SaVs，所以野猪可能是 SaVs 向猪群（反之亦然）传播的宿主，因为此前已在猪中检测到 GIII、GV 和 GVI SaVs。
