Kandel Mikhail E, Fernandes Daniel, Taylor Alison M, Shakir Haadi, Best-Popescu Catherine, Popescu Gabriel
Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, 61801.
Department of Biology, American University, Washington, District of Columbia, 20016.
Cytometry A. 2017 May;91(5):519-526. doi: 10.1002/cyto.a.23081. Epub 2017 Mar 13.
Due to the limitations of fluorescence imaging techniques, the study of intracellular cargo is typically restricted to two-dimensional analyses. To overcome low light levels and the risk of phototoxicity, we employ quantitative phase imaging, a family of full-field imaging techniques that measure the optical path length shift introduced by the specimen. Specifically, we use spatial light interference microscopy (SLIM) to study the transport of mass in whole tomographic volumes and show that a time-correlation technique, dispersion-relation phase spectroscopy (DPS), can be used to simultaneously assay the horizontal and vertical traffic of mass through a cell. To validate our method, we compare the traffic inside cell bodies and neuronal extensions, showing that the vertical transport of mass may prove a more sensitive and interesting metric than similar measurements limited to a 2D, horizontal plane. © 2017 International Society for Advancement of Cytometry.
由于荧光成像技术的局限性，细胞内货物的研究通常局限于二维分析。为了克服低光水平和光毒性风险，我们采用定量相位成像技术，这是一类全场成像技术，可测量样本引入的光程长度偏移。具体而言，我们使用空间光干涉显微镜（SLIM）研究整个断层体积中的物质运输，并表明一种时间相关技术，即色散关系相位光谱法（DPS），可用于同时测定物质通过细胞的水平和垂直运输。为了验证我们的方法，我们比较了细胞体和神经元延伸部分内的运输情况，结果表明，与仅限于二维水平面的类似测量相比，物质的垂直运输可能是一个更敏感且有趣的指标。© 2017国际细胞计量学促进协会。
