Medical Research Center for Prophylaxis and Health Protection in Industrial Workers, Ekaterinburg, 620014, Russia.
Institute of Industrial Ecology, The Urals Branch of the Russian Academy of Sciences, Ekaterinburg, Russia.
Food Chem Toxicol. 2019 Nov;133:110753. doi: 10.1016/j.fct.2019.110753. Epub 2019 Aug 7.
Isolated and combined damaging effects of PbO and CuO nanoparticles were estimated on an established line of human fibroblasts by a decrease in: (a) the cellular dehydrogenase activity (MTT Assay), (b) the ATP content (Luminescent Cell Viability Assay), (c) the cellular proliferation, viability, spreading, and attachment to substrate evaluated integrally by continuous impedance-based measurement of the Normalized Cell Index. Using all these indices, we demonstrate an explicit dependence of cell damage on the concentrations of both metal oxide nanoparticle (MeO-NP) species. This dependence is adequately approximated with a hyperbolic function. At equal exposure levels, PbO-NP and CuO-NP demonstrate quantitatively similar cytotoxicities. The same was observed previously for some non-specific in vivo toxicity measures. The combined in vitro cytotoxicity has also been described mathematically using the Response Surface Methodology and found to be represented by various types, thus corroborating, in this respect also, the findings of a previous animal experiment with the same MeO-NPs.
采用细胞脱氢酶活性（MTT 检测法）、三磷酸腺苷含量（发光细胞活力检测法）以及通过连续阻抗测量归一化细胞指数整体评估细胞增殖、活力、铺展和附着到基质的能力等指标，评估了 PbO 和 CuO 纳米粒子对已建立的人成纤维细胞系的单独和联合损伤效应。利用所有这些指标，我们证明了细胞损伤与两种金属氧化物纳米颗粒（MeO-NP）浓度之间存在明显的相关性。这种相关性可以用双曲线函数很好地近似。在相同的暴露水平下，PbO-NP 和 CuO-NP 表现出定量相似的细胞毒性。先前在一些非特异性体内毒性测量中也观察到了同样的情况。通过响应面法也对体外联合细胞毒性进行了数学描述，并发现它由各种类型表示，因此在这方面也证实了之前用相同 MeO-NP 进行的动物实验的结果。
