Department of Chemistry, Punjabi University, Patiala, 147002, Punjab, India.
Department of Chemistry, Punjabi University, Patiala, 147002, Punjab, India.
Environ Res. 2019 Sep;176:108580. doi: 10.1016/j.envres.2019.108580. Epub 2019 Jul 8.
A novel and porous molecularly imprinted polymer (PMIP) was synthesized and used as a solid-phase extraction adsorbent for preconcentration of carbamazepine (CBZ) prior to its quantitation by high-performance liquid chromatography (HPLC) in various sample forms (e.g., drinking water, river water, hospital wastewater, and pharmaceuticals). PMIP-CBZ was applied to a polymerization process in which polystyrene spheres were coated with a silica layer. Removal of polystyrene spheres and formation of porous silica facilitated the recovery of CBZ (99.4%) during the extraction process. Site accessibility to the surface of PMIP-CBZ increased the density of high-recognition sites. PMIP-CBZ was characterized by Fourier-transform infrared spectroscopy and scanning electron microscopy. The key variables influencing the extraction efficiency of PMIP (e.g., adsorbent loading, eluent type, eluent volume, reusability of the adsorbent, and cross-reactivity) were optimized. The optimized protocol was successfully employed to quantify CBZ with limit of detection and limit of quantification as 0.082 and 0.270 ng/mL, respectively (linear detection range: 0.5-250 ng/mL and a relative standard deviation: < 5%). Use of the PMIP adsorbent resulted in a sensitive and stable method for efficiently quantitation of CBZ from various real sample matrices.
一种新型多孔分子印迹聚合物（PMIP）被合成并用作固相萃取吸附剂，用于高效液相色谱（HPLC）在各种样品形式（如饮用水、河水、医院废水和药物）之前对卡马西平（CBZ）进行预浓缩。PMIP-CBZ 被应用于聚合过程中，其中聚苯乙烯球被涂覆有一层二氧化硅。去除聚苯乙烯球并形成多孔二氧化硅有助于在提取过程中回收 CBZ（99.4%）。PMIP-CBZ 的表面可接近性增加了高识别位点的密度。PMIP-CBZ 通过傅里叶变换红外光谱和扫描电子显微镜进行了表征。优化了影响 PMIP 萃取效率的关键变量（例如，吸附剂负载量、洗脱液类型、洗脱液体积、吸附剂的可重复使用性和交叉反应性）。优化的方案成功地用于定量 CBZ，检出限和定量限分别为 0.082 和 0.270ng/mL（线性检测范围：0.5-250ng/mL，相对标准偏差：<5%）。使用 PMIP 吸附剂可从各种实际样品基质中高效定量 CBZ，从而得到一种灵敏且稳定的方法。
