Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
BGI-Shenzhen, Beishan Industrial Zone, Shenzhen 518083, China; China National GeneBank, BGI-Shenzhen, Jinsha Road, Shenzhen 518120, China; BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China.
Syst Appl Microbiol. 2019 Sep;42(5):126004. doi: 10.1016/j.syapm.2019.126004. Epub 2019 Jul 30.
Two novel strains, designated 11W25H-1 and 8H24J-4-2, were isolated from surface-sterilized plant tissues collected from the Taklamakan Desert in the Xinjiang Uygur Autonomous Region, China. The strains were characterized by a polyphasic approach in order to clarify their taxonomic positions. They were Gram-stain positive, aerobic, non-motile, non-spore-forming and rod-shaped. The 16S rRNA gene sequences of the strains showed highest similarities with Labedella gwakjiensis KCTC 19176 (99.2% and 98.9%, respectively) and Labedella endophytica CPCC 203961 (98.9% and 99.0%, respectively). The sequence similarity between strains 11W25H-1 and 8H24J-4-2 was 99.4%. Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that the two strains belonged to the genus Labedella and formed a separate cluster from the closest species L. gwakjiensis KCTC 19176 and L. endophytica CPCC 203961. Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly separated the strains from each other and from the other species of the genus Labedella with values below the thresholds for species delineation. The two strains showed chemotaxonomic characteristics and phenotypic properties in agreement with the description of the genus Labedella and also confirmed the differentiation from the closest species. The data demonstrated that strains 11W25H-1 and 8H24J-4-2 represented two novel species of the genus Labedella, for which the names Labedella phragmitis sp. nov. (type strain 11W25H-1=JCM 33144=CGMCC 1.16700) and Labedella populi sp. nov. (type strain 8H24J-4-2=JCM 33143=CGMCC 1.16697) are proposed.
两株新的菌株，分别命名为 11W25H-1 和 8H24J-4-2，从中国新疆塔克拉玛干沙漠采集的表面消毒植物组织中分离出来。为了明确它们的分类地位，采用多相分类法对这些菌株进行了特征描述。它们革兰氏染色阳性，需氧，不运动，不产芽孢，呈杆状。菌株的 16S rRNA 基因序列与 Labedella gwakjiensis KCTC 19176（分别为 99.2%和 98.9%）和 Labedella endophytica CPCC 203961（分别为 98.9%和 99.0%）的相似度最高。菌株 11W25H-1 和 8H24J-4-2 之间的序列相似度为 99.4%。基于 16S rRNA 基因序列和单拷贝系统发育标记基因（pMGs）的系统发育分析表明，这两株菌属于 Labedella 属，与最接近的物种 L. gwakjiensis KCTC 19176 和 L. endophytica CPCC 203961 形成一个独立的聚类。基因组分析，包括平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH），清楚地将这两个菌株彼此区分开来，与 Labedella 属的其他物种也有明显的区分，这些值都低于物种划分的阈值。这两个菌株表现出化学分类特征和表型特性，与 Labedella 属的描述一致，也证实了与最接近的物种的分化。这些数据表明，菌株 11W25H-1 和 8H24J-4-2 代表了 Labedella 属的两个新种，分别命名为 Labedella phragmitis sp. nov.（模式菌株 11W25H-1=JCM 33144=CGMCC 1.16700）和 Labedella populi sp. nov.（模式菌株 8H24J-4-2=JCM 33143=CGMCC 1.16697）。
