Laboratory of Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, National Center for Children's Health, Beijing, 100045, China.
Beijing Key Laboratory of Antimicrobial Agents, Department of Microbial Chemistry, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China.
Antonie Van Leeuwenhoek. 2021 Oct;114(10):1657-1667. doi: 10.1007/s10482-021-01631-6. Epub 2021 Aug 2.
A novel actinobacterial strain, designated 10F1D-1, was isolated from soil sample collected from Futian mangrove nature reserve, China using of the in situ cultivation technique. Preliminary analysis based on the 16S rRNA gene sequence revealed that strain 10F1D-1 was the member of genus Schumannella with sharing highest sequence similarity (99.7%) to Schumannella luteola DSM 23141. Phylogenetic analyses based on 16S rRNA gene sequences and core proteome consistently exhibited that strain 10F1D-1 formed a monophyletic clade with Schumannella luteola DSM 23141. Comparative genomic analyses clearly separated strain 10F1D-1 from the only species of the genus Schumannella based on average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) values below the thresholds for species delineation. The genome of strain 10F1D-1 contains the biosynthetic gene clusters for osmoprotectants to adapt to the salt environment of mangrove. Strain 10F1D-1 also contains the biosynthetic gene clusters for bioactive compounds as secondary metabolites. On the basis of the polyphasic analysis, strain 10F1D-1 is considered to represent a novel species of the genus Schumannella, for which the name Schumannella soli sp. nov. (type strain 10F1D-1 = CGMCC1.16699 = JCM 33146) is proposed.
一株新型放线菌菌株，命名为 10F1D-1，是从中国福田红树林自然保护区的土壤样本中，采用原位培养技术分离得到的。基于 16S rRNA 基因序列的初步分析表明，菌株 10F1D-1 是舒曼氏菌属的成员，与舒曼氏菌 DSM 23141 的序列相似性最高（99.7%）。基于 16S rRNA 基因序列和核心蛋白质组的系统发育分析表明，菌株 10F1D-1 与舒曼氏菌 DSM 23141 形成一个单系分支。比较基因组分析清楚地将菌株 10F1D-1 与舒曼氏菌属的唯一物种基于平均核苷酸同一性 (ANI)、平均氨基酸同一性 (AAI) 和数字 DNA-DNA 杂交 (dDDH) 值从物种界限上区分开来。菌株 10F1D-1 的基因组包含适应红树林盐环境的渗透保护剂生物合成基因簇。菌株 10F1D-1 还包含生物活性化合物作为次生代谢物的生物合成基因簇。基于多相分析，菌株 10F1D-1 被认为代表舒曼氏菌属的一个新种，命名为舒曼氏菌土壤亚种（Schumannella soli sp. nov.）。（模式菌株 10F1D-1=CGMCC 1.16699=JCM 33146）。
