El Saftawy Enas A, Amin Noha M, Hamed Dina H, Elkazazz Aly, Adel Sherihan
1Medical Parasitology Department, College of Medicine, Cairo University, Cairo, Egypt.
Medical Parasitology Department, College of Medicine, Armed Forces College of Medicine, Cairo, Egypt.
J Parasit Dis. 2019 Sep;43(3):443-451. doi: 10.1007/s12639-019-01108-4. Epub 2019 Mar 30.
is highly prevalent with respiratory allergies among Egyptian children. Yet, little is known about the possible immunological relationship. Aims of this study were to measure complement-3 (C-3), total and specific IgE to intestinal allergens in patients' serum regarding the identified genotypes. In a cross-sectional study, three hundred children (150 asthmatics and 150 non asthmatics) participated in the study from both sexes, mean age 7.5 ± SD (3-4) years after a questionnaire administration. PCR-based genotyping of selective in vitro cultivation was performed. C-3, total and specific IgE were all measured in patients' serum utilizing ELISA. Blastocystosis was detected in 100 out of 300 children, 65 (43.3%) out of 150 asthmatics and 35 (23.3%) out of 150 non-asthmatics. Vacuolar forms were the most prevalent in both direct wet mount and stool cultures. Forty (61.5%) out 65 asthmatics and 5 (14.2%) out of 35 non-asthmatics were ≥ 5 organisms/HPF. Sex and irritable bowel disease were statistically insignificant ( value < 0.05). Urticaria was coincided in 15.4% of asthmatics and 8.6% of non-asthmatics. Of 100 cases of blastocystosis, eighty-four were genotype-3 and sixteen were genotype-4. Out of these, 55 cases of genotype-3 and 6 cases of genotype-4 were asthmatics. Positive C-3 serum levels were in 46 (54.81%) of genotype-3 and 2 (12.5%) of genotype-4. High total IgE levels in 30 (35.7%) out of 84 cases of genotype-3 and 4 (25%) out of 16 cases of genotype-4. Positive specific IgE was in 25 (29.8%) of genotype-3 and 3 (18.75%) of genotype-4. Genotype-3 was of higher infection intensity ( value = 0.0001). In conclusion, possess a hidden allergy triggering impact that can be obscured by simultaneous high (total and specific) IgE levels towards specific common intestinal allergens. Blastocystosis induces allergy by increasing C-3 serum levels in a genotype-dependent manner being higher in genotype-3. Virulence of genotype-3 seems to stand beyond increased parasite intensity and wide absorption of intestinal allergens that indirectly elevate IgE serum levels.
在埃及儿童中，呼吸道过敏非常普遍。然而，关于可能的免疫关系却知之甚少。本研究的目的是测量患者血清中补体3（C-3）、针对肠道过敏原的总IgE和特异性IgE，以确定相关基因型。在一项横断面研究中，300名儿童（150名哮喘患者和150名非哮喘患者）参与了研究，他们来自不同性别，在进行问卷调查后，平均年龄为7.5±标准差（3 - 4）岁。对选择性体外培养物进行了基于PCR的基因分型。利用酶联免疫吸附测定法（ELISA）测量患者血清中的C-3、总IgE和特异性IgE。在300名儿童中，有100名检测出芽囊原虫感染，其中150名哮喘患者中有65名（43.3%），150名非哮喘患者中有35名（23.3%）。在直接湿片和粪便培养中，空泡型最为常见。65名哮喘患者中有40名（61.5%），35名非哮喘患者中有5名（14.2%）每高倍视野（HPF）的虫体≥5个。性别和肠易激综合征在统计学上无显著差异（p值<0.05）。哮喘患者中有15.4%、非哮喘患者中有8.6%同时患有荨麻疹。在100例芽囊原虫感染病例中，84例为基因型3，16例为基因型4。其中，基因型3的55例和基因型4的6例为哮喘患者。基因型3患者的C-3血清水平呈阳性的有46例（54.81%），基因型4患者中有2例（12.5%）。基因型3的84例中有30例（35.7%）总IgE水平高，基因型4的16例中有
