Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892-0520, USA.
J Biomol NMR. 2019 Sep;73(8-9):461-469. doi: 10.1007/s10858-019-00244-6. Epub 2019 Aug 12.
A brief overview of theoretical and experimental aspects of the Dark state Exchange Saturation Transfer (DEST) and lifetime line broadening ([Formula: see text]) NMR methodologies is presented from a physico-chemical perspective. We describe how the field-dependence of [Formula: see text] can be used for determining the exchange regime on the transverse spin relaxation time-scale. Some limitations of DEST/[Formula: see text] methodology in applications to molecular systems with intermediate molecular weights are discussed, and the means of overcoming these limitations via the use of closely related exchange NMR techniques is presented. Finally, several applications of DEST/[Formula: see text] methodology are described from a methodological viewpoint, with an emphasis on providing examples of how kinetic and relaxation parameters of exchange can be reliably extracted from the experimental data in each particular case.
从物理化学的角度介绍了暗态交换饱和转移（DEST）和寿命线展宽([Formula: see text])NMR 方法的理论和实验方面的简要概述。我们描述了如何根据[Formula: see text]的场依赖性来确定横向自旋弛豫时间尺度上的交换状态。讨论了 DEST/[Formula: see text]方法在中等分子量分子体系中的应用的一些局限性，并提出了通过使用密切相关的交换 NMR 技术来克服这些局限性的方法。最后，从方法学的角度描述了 DEST/[Formula: see text]方法的几个应用，重点介绍了如何在每种特定情况下从实验数据中可靠地提取交换的动力学和弛豫参数的示例。
