Kayacan Yildirim, Çetinkaya Ayhan, Yazar Hayrullah, Makaracı Yücel
Yasar Dogu Faculty of Sports Sciences, Ondokuz Mayıs University, Samsun, Turkey.
Faculty of Medicine, Department of Physiology, Abant Izzet Baysal University, Bolu, Turkey.
Arch Physiol Biochem. 2021 Dec;127(6):504-508. doi: 10.1080/13813455.2019.1651868. Epub 2019 Aug 14.
The aim of this study was to investigate the effect of different intensity treadmill exercises on the thiol disulphide homeostasis which is a new marker of oxidative stress in rats. Male albino Wistar rats were randomly divided into four groups as follows: control (CNT), low (LEx), moderate (MEx) and high-intensity exercise (HEx) group. Exercise was performed for 4 weeks. Following completion of the experimental protocol, serum total thiol, native thiol and disulphide concentrations were determined using a novel automated measurement method. Additionally, dynamic disulphide status, reduced thiol, oxidised thiol and thiol oxidation reduction percentage ratios were compared among the groups. Disulphide levels were significantly lower in MEx group and highest in CNT group ( = .047). The lowest oxidised thiol and the highest reduced thiol were determined in CNT group ( = .086; = .083). These findings indicate that moderate-intensity exercise is more effective in reducing oxidative stress than low and high-intensity exercise.
本研究旨在探讨不同强度跑步机运动对大鼠硫醇-二硫化物稳态的影响，硫醇-二硫化物稳态是氧化应激的一个新标志物。雄性白化Wistar大鼠被随机分为四组：对照组（CNT）、低强度运动组（LEx）、中等强度运动组（MEx）和高强度运动组（HEx）。运动持续4周。实验方案完成后，采用一种新型自动测量方法测定血清总硫醇、天然硫醇和二硫化物浓度。此外，还比较了各组之间的动态二硫化物状态、还原型硫醇、氧化型硫醇和硫醇氧化还原百分比。二硫化物水平在MEx组显著低于CNT组（=0.047）。在CNT组中，氧化型硫醇最低，还原型硫醇最高（=0.086；=0.083）。这些发现表明，中等强度运动在降低氧化应激方面比低强度和高强度运动更有效。
