Deniz Cigdem Damla, Aktan Meryem, Erel Ozcan, Gurbilek Mehmet, Koc Mehmet
Department of Medical Biochemistry, Meram School of Medicine, Necmettin Erbakan University, Konya, Turkey.
Department of Radiation Oncology, Meram School of Medicine, Necmettin Erbakan University, Konya, Turkey.
J Radiat Res. 2019 Jan 1;60(1):23-28. doi: 10.1093/jrr/rry083.
Ionizing radiation-induced free radicals cause functional and structural harmful effects. Thiol, an important antioxidant, plays a major role in the eradication of reactive oxygen molecules. Thiol/disulphide homeostasis is a marker of oxidative stress. The objective of this study was to assess the potential radioprotective effects of thymoquinone (TQ) on the dynamic thiol/disulphide homeostasis of rats receiving total-body irradiation (IR). Twenty-two rats were divided into three groups to test the radioprotective effectiveness of TQ. The sham control group did not receive TQ or IR. The IR group received only total-body IR. The TQ + IR group received IR plus TQ. Following IR, blood samples were taken. The thiol/disulphide homeostasis parameters were analysed by a newly established method. In the IR group, native thiol and the native thiol/total thiol ratio were significantly decreased (P = 0.003 and P = 0.003, respectively), whereas the disulphide/native thiol and disulphide/total thiol ratios were significantly increased when compared with those of the sham control group (P = 0.003 and P = 0.003, respectively). In the TQ + IR group, the mean disulphide, native thiol and total thiol levels and the disulphide/native thiol, disulphide/total thiol and native thiol/total thiol ratios were not found to be significantly different when compared with those of the sham control group (P > 0.05 for all). Thiol/disulphide homeostasis was found to be disturbed after IR exposure. The results showed that TQ had antioxidant effects and reduced the IR-induced oxidative stress, which was demonstrated through the dynamic thiol/disulphide homeostasis. Thus, the use of TQ before radiation treatment helped protect the rats from oxidant side effects.
电离辐射诱导产生的自由基会造成功能和结构上的有害影响。硫醇作为一种重要的抗氧化剂，在清除活性氧分子过程中发挥着主要作用。硫醇/二硫键稳态是氧化应激的一个标志物。本研究的目的是评估胸腺醌（TQ）对接受全身照射（IR）大鼠的动态硫醇/二硫键稳态的潜在辐射防护作用。将22只大鼠分为三组以测试TQ的辐射防护效果。假手术对照组既不接受TQ也不接受IR。IR组仅接受全身照射。TQ + IR组接受照射加TQ。照射后采集血样。采用一种新建立的方法分析硫醇/二硫键稳态参数。在IR组中，与假手术对照组相比，天然硫醇和天然硫醇/总硫醇比值显著降低（分别为P = 0.003和P = 0.003），而二硫键/天然硫醇和二硫键/总硫醇比值显著升高（分别为P = 0.003和P = 0.003）。在TQ + IR组中，与假手术对照组相比，二硫键、天然硫醇和总硫醇的平均水平以及二硫键/天然硫醇、二硫键/总硫醇和天然硫醇/总硫醇比值均未发现有显著差异（所有P > 0.05）。发现照射后硫醇/二硫键稳态受到干扰。结果表明，TQ具有抗氧化作用，并减轻了照射诱导的氧化应激，这通过动态硫醇/二硫键稳态得以证明。因此，在放射治疗前使用TQ有助于保护大鼠免受氧化副作用的影响。
