Department of Plant Biology, University of Illinois, 1201 W Gregory Dr., Urbana, Illinois 61801.
Center for Genomics and Systems Biology, New York University, 12 Waverly Place, New York, New York 10001.
Plant Physiol. 2019 Nov;181(3):1371-1388. doi: 10.1104/pp.19.00685. Epub 2019 Aug 13.
Plant responses to multiple environmental stimuli must be integrated to enable them to adapt their metabolism and development. Light and nitrogen (N) are two such stimuli whose downstream signaling pathways must be intimately connected to each other to control plant energy status. Here, we describe the functional role of the WRKY1 transcription factor in controlling genome-wide transcriptional reprogramming of Arabidopsis () leaves in response to individual and combined light and N signals. This includes a cross-regulatory network consisting of 724 genes regulated by WRKY1 and involved in both N and light signaling pathways. The loss of gene function has marked effects on the light and N response of genes involved in N uptake and assimilation (primary metabolism) as well as stress response pathways (secondary metabolism). Our results at the transcriptome and at the metabolite analysis level support a model in which WRKY1 enables plants to activate genes involved in the recycling of cellular carbon resources when light is limiting but N is abundant and upregulate amino acid metabolism when both light and N are limiting. In this potential energy conservation mechanism, WRKY1 integrates information about cellular N and light energy resources to trigger changes in plant metabolism.
植物对多种环境刺激的反应必须进行整合，以使它们能够调节其新陈代谢和发育。光和氮（N）是两种这样的刺激物，其下游信号通路必须紧密相连，以控制植物的能量状态。在这里，我们描述了 WRKY1 转录因子在控制拟南芥（）叶片对单个和组合的光和 N 信号的全基因组转录重编程中的功能作用。这包括一个由 WRKY1 调节的 724 个基因的交叉调控网络，这些基因参与 N 和光信号通路。基因功能的丧失对参与 N 吸收和同化（初级代谢）以及应激反应途径（次生代谢）的基因的光和 N 反应有显著影响。我们在转录组和代谢物分析水平上的结果支持这样一种模型，即 WRKY1 使植物能够在光有限但 N 丰富时激活涉及细胞碳资源再循环的基因，并在光和 N 都有限时上调氨基酸代谢。在这种潜在的能量守恒机制中，WRKY1 整合了有关细胞 N 和光能资源的信息，以触发植物代谢的变化。
