Hatz R A, Bloch K J, Harmatz P R, Gonnella P A, Ariniello P D, Walker W A, Kleinman R E
Department of Pediatrics, Harvard Medical School, Boston, Massachusetts.
Gastroenterology. 1990 Apr;98(4):894-900. doi: 10.1016/0016-5085(90)90013-q.
The capacity of the stomach to participate in anaphylaxis induced by the hapten N,N'-di-2,4,dinitrophenyllysine (di-DNP-lysine) was examined in BDF1 female mice immunized with dinitrophenylated Ascaris suum extract. Immunized animals underwent laparotomy and nontraumatic pyloric occlusion using a microvascular clamp. Following wound closure, animals were gavage-fed ovalbumin together with di-DNP-lysine. Other mice were subjected to systemic anaphylaxis by intravenous injection of di-DNP-lysine administered 1 min after gavage feeding of ovalbumin. The intravenous and intragastric administration of di-DNP-lysine led to a sixfold or greater increase in serum immunoreactive ovalbumin. Examination of 1-micron sections of gastric tissue from DNP-Asc-immunized and unimmunized mice showed an intact mucosal and submucosal architecture. A 75% increase in the number of mast cells below the muscularis mucosa was seen in immunized compared with unimmunized BDF1 mice. Gastric tissue sections from immunized mice challenged orally or intravenously with di-DNP-lysine showed compaction of erythrocytes in blood vessels, degranulation of mast cells, degenerative changes in the gastric epithelium, expulsion of mucus from gastric glands, and edema in the lamina propria. The present model may be useful for further defining the consequences of anaphylaxis on the development of immune responses to dietary antigens.
在经二硝基苯基化猪蛔虫提取物免疫的BDF1雌性小鼠中，检测了胃参与由半抗原N，N'-二-2,4-二硝基苯基赖氨酸（二-DNP-赖氨酸）诱导的过敏反应的能力。对免疫动物进行剖腹手术，并用微血管夹进行非创伤性幽门闭塞。伤口闭合后，给动物灌喂卵清蛋白和二-DNP-赖氨酸。其他小鼠在灌喂卵清蛋白1分钟后通过静脉注射二-DNP-赖氨酸进行全身性过敏反应。二-DNP-赖氨酸的静脉内和胃内给药导致血清免疫反应性卵清蛋白增加六倍或更多。对经DNP-猪蛔虫免疫和未免疫小鼠的胃组织1微米切片检查显示，黏膜和黏膜下层结构完整。与未免疫的BDF1小鼠相比，免疫小鼠黏膜肌层下方肥大细胞数量增加了75%。经口服或静脉注射二-DNP-赖氨酸攻击的免疫小鼠的胃组织切片显示血管内红细胞凝聚、肥大细胞脱颗粒、胃上皮细胞变性改变、胃腺黏液排出以及固有层水肿。本模型可能有助于进一步明确过敏反应对饮食抗原免疫反应发展的影响。
