Oliveira Simone Q, Kratz Jadel M, Chaves Vitor C, Guimarães Tatiana R, Costa Danielle T M, Dimitrakoudi Sapfo, Vontzalidou Argyro, Bordignon Sérgio A L, Simionato Cesar P, Steindel Mário, Reginatto Flávio H, Simões Cláudia M O, Schenkel Eloir P
Programa dePós-Graduação em Farmácia, Universidade Federal de Santa Catarina, Rua Delfino Conti, s/n, Campus Universitário, Trindade, 88040-900 Florianópolis, SC, Brazil.
Programa de Pós-Graduação em Biotecnologia e Biociências, Universidade Federal de Santa Catarina, Rua Delfino Conti, s/n, Campus Universitário, Trindade, 88040-900 Florianópolis, SC, Brazil.
An Acad Bras Cienc. 2019 Aug 12;91(3):e20180621. doi: 10.1590/0001-3765201920180621.
Aristolochia triangularis Cham., is one of the most frequently used medicinal plant in Southern Brazil. Preparations containing the leaves and/or stems are traditionally used as anti-inflammatory, diuretic, as well as antidote against snakebites. This study screened A. triangularis extracts, fractions and isolated compounds for different bioactivities. A weak antiproliferative activity against human lung cancer cell line (A549) was observed only for chloroform fraction obtained from stems (CFstems - CC50: 2.93 µg/mL). Also, a moderate antimicrobial activity against Staphylococcus aureus was detected just for chloroform fraction obtained from leaves (CFleaves -13-16 mm inhibition zone). Additionally, two semi-purified fractions (CFstems-4 and CFleaves-4) selectively inhibited HSV-1 replication (IC50 values of 0.40 and 2.61 µg/mL, respectively), while only CFleaves showed promising results against Leishmania amazonensis. Fractionation of extracts resulted in the isolation of one neolignan (-) cubebin and one lignan (+) galbacin. However, these compounds are not responsible for the in vitro bioactivities herein detected. The presence of aristolochic acid I and aristolochic acid II in the crude ethanol extract of stems (CEEstems) and leaves (CEEleaves) was also investigated. The HPLC analysis of these extracts did not display any peak with retention time or UV spectra comparable to aristolochic acids I and II.
三角叶马兜铃是巴西南部最常用的药用植物之一。含有叶片和/或茎干的制剂传统上被用作抗炎、利尿以及蛇咬伤的解毒剂。本研究对三角叶马兜铃的提取物、馏分和分离出的化合物进行了不同生物活性的筛选。仅从茎干中获得的氯仿馏分（CFstems - CC50：2.93 μg/mL）对人肺癌细胞系（A549）表现出微弱的抗增殖活性。此外，仅从叶片中获得的氯仿馏分（CFleaves - 13 - 16毫米抑菌圈）对金黄色葡萄球菌有中度抗菌活性。另外，两个半纯化馏分（CFstems - 4和CFleaves - 4）选择性抑制单纯疱疹病毒1型（HSV - 1）复制（IC50值分别为0.40和2.61 μg/mL），而只有CFleaves对亚马逊利什曼原虫显示出有前景的结果。提取物的分馏导致分离出一种新木脂素（-）荜澄茄素和一种木脂素（+）高儿茶素。然而，这些化合物并非本文所检测到的体外生物活性的原因。还对茎干（CEEstems）和叶片（CEEleaves）的粗乙醇提取物中马兜铃酸I和马兜铃酸II的存在情况进行了研究。这些提取物的高效液相色谱分析未显示出任何保留时间或紫外光谱与马兜铃酸I和II相当的峰。
