Martín A M, Rodríguez J, Canut A, Dovigo C A
Departamento de Microbiología, Hospital Clínico Universitario, Universidad de Salamanca.
Enferm Infecc Microbiol Clin. 1992 Jan;10(1):39-42.
An evaluation is made of the utility for the diagnosis of giardiasis of an enzyme immunoassay (EIA) that detects the GSA65 specific antigen in feces, as compared with the formalin-ether sedimentation test. Within the context of a longitudinal study on intestinal parasitization, 147 fecal samples from children attending 7 day care centres in the city of Salamanca were studied. The feces were examined a few hours after collection by the sedimentation technique and conserved in 10% formol. They were later studied by the EIA method. G. intestinalis was detected by microscopy and/or EIA in 26 of the 147 fecal samples (17.7%). Both assays were positive in 22 of the 26 cases detected (84.6%) and the remaining 4 cases were positive only by EIA (4/26; 15.4%). The EIA assay, using visual reading and very simple to implement, showed sensitivity, specificity, positive predictive and negative predictive values of 100, 96.9, 84.6 and 100%, respectively.
对一种检测粪便中GSA65特异性抗原的酶免疫测定法（EIA）在贾第虫病诊断中的效用进行了评估，并与福尔马林-乙醚沉淀试验进行了比较。在一项关于肠道寄生虫感染的纵向研究中，对来自萨拉曼卡市7个日托中心儿童的147份粪便样本进行了研究。粪便在采集后数小时通过沉淀技术进行检查，并保存在10%的甲醛中。随后采用EIA方法进行研究。在147份粪便样本中的26份（17.7%）通过显微镜检查和/或EIA检测到肠道贾第虫。在检测出的26例病例中，两种检测方法均为阳性的有22例（84.6%），其余4例仅EIA检测为阳性（4/26；15.4%）。EIA检测采用目视读数，实施非常简单，其敏感性、特异性、阳性预测值和阴性预测值分别为100%、96.9%、84.6%和100%。
