Ortiz-Mendoza Nancy, San Miguel-Chávez Rubén, Martínez-Gordillo Martha Juana, Basurto-Peña Francisco Alberto, Palma-Tenango Mariana, Aguirre-Hernández Eva
Laboratorio de Productos Naturales, Departamento de Ecología y Recursos Naturales, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad de México 04510, Mexico.
Posgrado en Ciencias Biológicas, Unidad de Posgrado, Edificio D, 1° Piso, Circuito de Posgrados, Ciudad Universitaria Coyoacán, Ciudad de México 04510, Mexico.
Metabolites. 2023 Jul 20;13(7):866. doi: 10.3390/metabo13070866.
Zucc. (Lamiaceae) is endemic to Oaxaca, Mexico, and is known for its analgesic properties. Terpenoids and phenolic compounds with antinociceptive potential have been characterised from this species. The aim of this research was to determine the variation in terpenoids and flavonoids in ethyl acetate extracts of collected from ten different localities, as well as to evaluate the antinociceptive effect between plants with higher and lower contents of these secondary metabolites. Quantification of compounds was performed via HPLC-DAD, whereas in vivo evaluation of the antinociceptive effect was performed via formalin test. The results showed that the most abundant groups of metabolites are oleanolic acid (89.60-59.20 µg/mg), quercetin (34.81-16.28 µg/mg), catechin (11.30-9.30 µg/mg), and 7-keto-neoclerodan-3,13-dien-18,19:15,16-diolide (7-keto) (8.01-4.76 µg/mg). Principal component and canonical correspondence analysis showed that the most contrasting localities in terms of compound content and climatic variables are Miahuatlán and Santiago Huauclilla. The differences in metabolite content between the two locations did not affect the antinociceptive effects evaluated at a dose of 300 mg/kg, p.o. In conclusion, the results indicate that is effective in relieving pain, regardless of the site of collection, reinforcing its traditional use as analgesic.
祖克（唇形科）原产于墨西哥瓦哈卡州，以其止痛特性而闻名。已从该物种中鉴定出具有抗伤害感受潜力的萜类化合物和酚类化合物。本研究的目的是确定从十个不同地点采集的乙酸乙酯提取物中萜类化合物和黄酮类化合物的变化，以及评估这些次生代谢物含量较高和较低的植物之间的抗伤害感受效果。通过高效液相色谱 - 二极管阵列检测法（HPLC - DAD）对化合物进行定量，而通过福尔马林试验对抗伤害感受效果进行体内评估。结果表明，最丰富的代谢物组为齐墩果酸（89.60 - 59.20 µg/mg）、槲皮素（34.81 - 16.28 µg/mg）、儿茶素（11.30 - 9.30 µg/mg）和7 - 酮 - 新克罗丹 - 3,13 - 二烯 - 18,19:15,16 - 二内酯（7 - 酮）（8.01 - 4.76 µg/mg）。主成分分析和典范对应分析表明，在化合物含量和气候变量方面差异最大的地点是米亚特拉恩和圣地亚哥·瓦乌克利利亚。两个地点之间代谢物含量的差异并未影响以300 mg/kg口服剂量评估的抗伤害感受效果。总之，结果表明，无论采集地点如何，祖克在缓解疼痛方面均有效，这强化了其作为止痛药的传统用途。
