Déciga-Campos Myrna, Mata Rachel, Rivero-Cruz Isabel
Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina del Instituto Politécnico Nacional, Ciudad de México 11340, México.
Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México 04510, México.
Biomed Pharmacother. 2017 May;89:933-938. doi: 10.1016/j.biopha.2017.02.096. Epub 2017 Mar 8.
This work evaluates the potential antinociceptive activity of Dysphania graveolens, traditional medicinal plant used in Mexico to treat stomach pain. A CHCl-MeOH extract, infusion and essential oil from aerial parts of Dysphania graveolens were evaluated in hot plate and writhing tests in mice. The metabolites pinostrobin, pinocembrin and chrysin were isolated from the Dysphania graveolens infusion; next, they were evaluated in both nociceptive tests. To confirm the antinociceptive activity and explore the possible participation of opioid, GABA and serotonin receptors in the pharmacological mechanism, a formalin test was used. Oral administration of Dysphania graveolens CHCl-MeOH extract, infusion and essential oil (31-316mg/kg) produced an antinociceptive response to thermic and chemical algesic stimuli. Essential oil was the most active partition of this plant. In addition, the secondary metabolites pinostrobin, pinocembrin and chrysin possess a significant antinociceptive effect. This response was confirmed by the formalin test for the CHCl-MeOH extract of Dysphania graveolens and chrysin. In both cases, the antinociceptive activity was reverted in the presence of naltrexone, flumazenil and bicuculline antagonists. The 5-HT receptors did not participate in the antinociceptive response of this plant. The overall information tends to support the efficacy of Dysphania graveolens as an analgesic and its cultural use in abdominal pain.
这项研究评估了墨西哥用于治疗胃痛的传统药用植物腺毛杯苋的潜在镇痛活性。对腺毛杯苋地上部分的氯仿 - 甲醇提取物、浸剂和精油进行了小鼠热板法和扭体试验评估。从腺毛杯苋浸剂中分离出了代谢产物松属素、白杨素和白杨黄素；接下来，对它们进行了两种痛觉测试评估。为了确认镇痛活性并探究阿片类、GABA和5 - 羟色胺受体在药理机制中可能的参与情况，使用了福尔马林试验。口服腺毛杯苋氯仿 - 甲醇提取物、浸剂和精油（31 - 316毫克/千克）对热和化学性痛觉刺激产生了镇痛反应。精油是该植物中活性最强的组分。此外，次生代谢产物松属素、白杨素和白杨黄素具有显著的镇痛作用。腺毛杯苋氯仿 - 甲醇提取物和白杨黄素的福尔马林试验证实了这种反应。在这两种情况下，纳曲酮、氟马西尼和荷包牡丹碱拮抗剂存在时，镇痛活性均被逆转。5 - 羟色胺受体未参与该植物的镇痛反应。总体信息倾向于支持腺毛杯苋作为镇痛药的有效性及其在腹痛方面的传统应用。
