藻类藻蓝蛋白促进培养中的人类细胞生长。

Algal phycocyanins promote growth of human cells in culture.

作者信息

Shinohara K, Okura Y, Koyano T, Murakami H, Omura H

机构信息

Food Additive Laboratory, National Food Research Institute, Ibaraki, Japan.

出版信息

In Vitro Cell Dev Biol. 1988 Oct;24(10):1057-60. doi: 10.1007/BF02620882.

DOI:10.1007/BF02620882

PMID:3141367

Abstract

The growth-promoting substances in a non-dialyzable extract of Synechococcus elongatus var. on RPMI 8226 cells (a human myeloma cell line) were separated by gel filtration and ion exchange chromatography. By gel filtration with Sepharose 4B, the dialyzate was separated into two fractions. One fraction was green-colored (P-1) and the other was blue-colored (P-2). The P-2 fraction had a higher growth-promoting activity than P-1. By ion exchange chromatography, the P-2 fraction was separated into two blue-colored fractions of phycocyanin and allophycocyanin. Both biliproteins promoted the growth of RPMI 8226 cells; however, allophycocyanin was more active than phycocyanin.

摘要

通过凝胶过滤和离子交换色谱法，对细长聚球藻变种的不可透析提取物中促进RPMI 8226细胞（一种人骨髓瘤细胞系）生长的物质进行了分离。通过用琼脂糖4B进行凝胶过滤，透析液被分离成两个部分。一个部分呈绿色（P-1），另一个部分呈蓝色（P-2）。P-2部分的促生长活性高于P-1。通过离子交换色谱法，P-2部分被分离成藻蓝蛋白和别藻蓝蛋白两个蓝色部分。两种双蛋白均促进RPMI 8226细胞的生长；然而，别藻蓝蛋白比藻蓝蛋白更具活性。