Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA.
School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
STAR Protoc. 2021 Nov 5;2(4):100879. doi: 10.1016/j.xpro.2021.100879. eCollection 2021 Dec 17.
It can be challenging to maintain tissue integrity using established histology protocols. Here, we describe a protocol composed of Hartman's fixation, window technique, microwave-based tissue processing, optimized depigmentation, and antigen retrieval pretreatment. This is followed by the ViewRNA single-molecule fluorescence in situ hybridization and immunofluorescence techniques to optimize routine histological staining and molecular histology multiplexing assays. Our protocol is highly reproducible in any laboratory and may decrease animal usage and lab resource expenditure. For complete details on the use and execution of this protocol, please refer to Pang et al. (2021).
使用既定的组织学方案来维持组织完整性可能具有挑战性。在这里，我们描述了一个由 Hartman 固定、窗口技术、基于微波的组织处理、优化的脱色和抗原修复预处理组成的方案。随后，采用 ViewRNA 单分子荧光原位杂交和免疫荧光技术来优化常规组织学染色和分子组织学多重分析。我们的方案在任何实验室中都具有高度可重复性，并可能减少动物使用和实验室资源支出。有关该方案的使用和实施的完整详细信息，请参阅 Pang 等人（2021 年）。
