Department of Molecular Medicine, Sapienza University of Rome, Viale Regina Elena 291, 00161, Rome, Italy.
Istituto Italiano di Tecnologia, Center for Life Nano Science@Sapienza, Viale Regina Elena 291, 00161, Rome, Italy.
Nat Commun. 2019 Aug 15;10(1):3686. doi: 10.1038/s41467-019-11642-7.
In vivo liposomes, like other types of nanoparticles, acquire a totally new 'biological identity' due to the formation of a biomolecular coating known as the protein corona that depends on and modifies the liposomes' synthetic identity. The liposome-protein corona is a dynamic interface that regulates the interaction of liposomes with the physiological environment. Here we show that the biological identity of liposomes is clearly linked to their sequestration from peripheral blood mononuclear cells (PBMCs) of healthy donors that ultimately leads to removal from the bloodstream. Pre-coating liposomes with an artificial corona made of human plasma proteins drastically reduces capture by circulating leukocytes in whole blood and may be an effective strategy to enable prolonged circulation in vivo. We conclude with a critical assessment of the key concepts of liposome technology that need to be reviewed for its definitive clinical translation.
在体内，脂质体与其他类型的纳米颗粒一样，由于形成了一种称为蛋白质冠的生物分子涂层，从而获得了全新的“生物学身份”，这种蛋白质冠取决于并修饰了脂质体的合成身份。脂质体-蛋白质冠是一个动态界面，调节着脂质体与生理环境的相互作用。在这里，我们表明脂质体的生物学身份与其被健康供体外周血单核细胞（PBMCs）隔离明显相关，这最终导致其从血液中清除。用由人血浆蛋白制成的人工蛋白质冠预先包被脂质体，可大大减少循环白细胞在全血中的捕获，可能是实现体内延长循环的有效策略。最后，我们对脂质体技术的关键概念进行了批判性评估，这些概念需要在其明确的临床转化中进行回顾。
