基于 LED 的光声成像用于监测纤维蛋白支架中的血管生成。
LED-Based Photoacoustic Imaging for Monitoring Angiogenesis in Fibrin Scaffolds.
机构信息
Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan.
Department of Electronic Science and Engineering, Nanjing University, Nanjing, China.
出版信息
Tissue Eng Part C Methods. 2019 Sep;25(9):523-531. doi: 10.1089/ten.TEC.2019.0151. Epub 2019 Sep 11.
Abstract
Noninvasive imaging techniques provide insight into physiology that is complementary to tissue morphology obtained by invasive histology. Optical imaging techniques, such as laser speckle contrast analysis, are used to longitudinally evaluate vascularization. Despite their high spatial resolution, these techniques have a limited imaging depth. In this study, we demonstrate how a dual LED-based photoacoustic (PA) and ultrasound system can delineate changes in perfusion at depth within scaffolds containing basic fibroblast growth factor. Perfusion changes detected by PA corroborated with vessel density. PA imaging could be a noninvasive and sensitive method for evaluating vascularization at depth in larger constructs.
摘要
非侵入性成像技术提供了对生理学的深入了解，与通过侵入性组织学获得的组织形态互补。光学成像技术，如激光散斑对比分析，用于纵向评估血管生成。尽管这些技术具有较高的空间分辨率，但它们的成像深度有限。在这项研究中，我们展示了如何使用基于双 LED 的光声（PA）和超声系统来描绘含有碱性成纤维细胞生长因子的支架内深处的灌注变化。PA 检测到的灌注变化与血管密度相符。PA 成像可能是一种非侵入性和敏感的方法，可用于评估较大构建体深处的血管生成。
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