Center for International Research on Integrative Biomedical Systems, Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.
R&D Department 1, Screen Holdings Co., Ltd., 322 Furukawa-cho, Hazukashi, Fushimi-ku, Kyoto 612-8486, Japan.
Sci Rep. 2017 Feb 10;7:42426. doi: 10.1038/srep42426.
Three-dimensional (3D) in vitro microvasculature in a polydimethylsiloxane-based microdevice was developed as a physiologically relevant model of angiogenesis. The angiogenic process is monitored using stage-top optical coherence tomography (OCT). OCT allows non-invasive monitoring of the 3D structures of the prepared host microvasculature and sprouted neovasculature without fluorescence staining. OCT monitoring takes only a few minutes to scan through the several-millimetre scale range, which provides the advantage of rapid observation of living samples. The obtained OCT cross-sectional images capture 3D features of the angiogenic sprouting process and provide information on the dynamics of luminal formation. The stage-top system used in this study enables the observer to visualize the in vitro dynamics of 3D cultured cells simply and conveniently, offering an alternative monitoring method for studies on angiogenesis and providing quantitative information about vascular morphological changes.
我们开发了一种基于聚二甲基硅氧烷的三维（3D）体外微血管微器件，作为一种与生理相关的血管生成模型。使用顶置式光学相干断层扫描（OCT）监测血管生成过程。OCT 允许在不进行荧光染色的情况下，对制备的宿主微血管和新生成的血管进行非侵入性的 3D 结构监测。OCT 监测只需几分钟即可扫描数毫米的范围，这为快速观察活体样本提供了优势。获得的 OCT 横截面图像捕捉到血管生成发芽过程的 3D 特征，并提供关于管腔形成动力学的信息。本研究中使用的顶置系统使观察者能够简单方便地观察 3D 培养细胞的体外动力学，为血管生成研究提供了一种替代监测方法，并提供了有关血管形态变化的定量信息。
