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多官能植物应激调节剂的表观遗传沉默

Epigenetic silencing of a multifunctional plant stress regulator.

机构信息

Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, United States.

Genomic Analysis Laboratory, Salk Institute for Biological Studies, La Jolla, United States.

出版信息

Elife. 2019 Aug 16;8:e47835. doi: 10.7554/eLife.47835.

DOI:10.7554/eLife.47835
PMID:31418686
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6739875/
Abstract

The central regulator of the ethylene (ET) signaling pathway, which controls a plethora of developmental programs and responses to environmental cues in plants, is ETHYLENE-INSENSITIVE2 (EIN2). Here we identify a chromatin-dependent regulatory mechanism at requiring two genes: ETHYLENE-INSENSITIVE6 (EIN6), which is a H3K27me3 demethylase also known as (), and EIN6 ENHANCER (EEN), the homolog of the yeast INO80 chromatin remodeling complex subunit (). Strikingly, EIN6 (REF6) and the INO80 complex redundantly control the level and the localization of the repressive histone modification H3K27me3 and the histone variant H2A.Z at the 5' untranslated region (5'UTR) intron of . Concomitant loss of EIN6 (REF6) and the INO80 complex shifts the chromatin landscape at to a repressive state causing a dramatic reduction of expression. These results uncover a unique type of chromatin regulation which safeguards the expression of an essential multifunctional plant stress regulator.

摘要

乙烯（ET）信号通路的中央调节剂，控制着植物中大量的发育程序和对环境线索的反应，是 ETHYLENE-INSENSITIVE2（EIN2）。在这里，我们确定了一个需要两个基因的染色质依赖的调节机制：ETHYLENE-INSENSITIVE6（EIN6），它是一种 H3K27me3 去甲基化酶，也称为 （），和 EIN6 ENHANCER（EEN），酵母 INO80 染色质重塑复合物亚基 （）的同源物。引人注目的是，EIN6（REF6）和 INO80 复合物冗余地控制抑制性组蛋白修饰 H3K27me3 和组蛋白变体 H2A.Z 的水平和定位在 5'非翻译区（5'UTR）内含子的 。EIN6（REF6）和 INO80 复合物的同时缺失将 处的染色质景观转变为抑制状态，导致 表达的急剧减少。这些结果揭示了一种独特的染色质调控类型，它可以保护一种必需的多功能植物应激调节剂的表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/6216863fe2f7/elife-47835-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/bf2c774c1b9c/elife-47835-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/02129ca7ddf5/elife-47835-fig1-figsupp1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/935aef0a6b45/elife-47835-fig2-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/a98dd9af2184/elife-47835-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/cdb4a5279402/elife-47835-fig3-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/1f885ba28ae4/elife-47835-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/6ee0f8772071/elife-47835-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/5f3ff954d8bb/elife-47835-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/6216863fe2f7/elife-47835-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/bf2c774c1b9c/elife-47835-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/02129ca7ddf5/elife-47835-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/3805d8a45135/elife-47835-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/777624c67151/elife-47835-fig2-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/935aef0a6b45/elife-47835-fig2-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/a98dd9af2184/elife-47835-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/cdb4a5279402/elife-47835-fig3-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/1f885ba28ae4/elife-47835-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/6ee0f8772071/elife-47835-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/5f3ff954d8bb/elife-47835-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4e5/6739875/6216863fe2f7/elife-47835-fig5-figsupp1.jpg

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