Pier G B
Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115.
Rev Infect Dis. 1988 Jul-Aug;10 Suppl 2:S337-40. doi: 10.1093/cid/10.supplement_2.s337.
Cell-surface polysaccharide antigens of Pseudomonas aeruginosa are important determinants of pathogenicity and represent target antigens for the development of vaccines. Two major and distinct cell-surface polysaccharides are expressed by different strains of P. aeruginosa: the serotype determinant located on the O polysaccharide side chain of the lipopolysaccharide and mucoid exopolysaccharide (MEP), an antigen usually made by isolates of P. aeruginosa from patients with cystic fibrosis. The serotype determinant has been isolated in a high-molecular-weight, non-toxic, immunogenic form known as high-molecular-weight polysaccharide. High-molecular-weight polysaccharide is safe and immunogenic in humans and elicits T cell immunity to P. aeruginosa infection in animals. Purified MEP antigen has been shown to be immunogenic in animals, and patients with cystic fibrosis colonized with mucoid P. aeruginosa respond to MEP with the production of high-titered serum antibody. In spite of brisk immune responses of patients with cystic fibrosis to MEP, they are unable to deal with the chronic respiratory colonization by mucoid P. aeruginosa. An association has been established between the presence of opsonic-killing antibody to MEP and the lack of colonization of older patients with cystic fibrosis with P. aeruginosa.
铜绿假单胞菌的细胞表面多糖抗原是致病性的重要决定因素，也是疫苗开发的靶抗原。不同菌株的铜绿假单胞菌表达两种主要且不同的细胞表面多糖：位于脂多糖O多糖侧链上的血清型决定簇和黏液样胞外多糖（MEP），后者是通常由囊性纤维化患者分离出的铜绿假单胞菌菌株产生的一种抗原。血清型决定簇已被分离成一种高分子量、无毒、具有免疫原性的形式，即高分子量多糖。高分子量多糖在人体中安全且具有免疫原性，并能在动物体内引发针对铜绿假单胞菌感染的T细胞免疫。纯化的MEP抗原已被证明在动物中具有免疫原性，并且定植有黏液样铜绿假单胞菌的囊性纤维化患者对MEP产生反应，产生高滴度的血清抗体。尽管囊性纤维化患者对MEP有活跃的免疫反应，但他们无法应对黏液样铜绿假单胞菌的慢性呼吸道定植。已证实针对MEP的调理吞噬杀伤抗体的存在与老年囊性纤维化患者未被铜绿假单胞菌定植之间存在关联。
