Pier G B
Antibiot Chemother (1971). 1985;36:157-67.
Surface polysaccharide antigens, expressed by strains of P. aeruginosa, are readily available for interaction with the host immune systems. These interactions could potentially result in development of protective immunity against P. aeruginosa infections. Classic strains of P. aeruginosa isolated from immunocompromised hosts and the environment generally express cell surface LPS antigens containing the serotype determinant. Antibody to these determinants has clearly been shown to be protective in both animals and humans. Immunization with a nontoxic, high molecular weight polysaccharide fraction, obtained from the culture supernate of P. aeruginosa, results in the induction of high titered, functional antibody against the type specific determinant. In addition, experimental protocols in animals have shown that a T cell-mediated immunity against type specific antigens can also provide protective immunity. Although the role of T cell-mediated immunity in protection against P. aeruginosa infections is unclear, it may be important in augmenting antibody-mediated protection. P. aeruginosa isolates from CF patients generally do not express type-specific antigens. They do, however, express a different cell surface polysaccharide, MEP. Antibody to this material is made by the colonized CF host, but it clearly is not associated with protective immunity. Animal antibody raised to this material is able to mediate opsonic killing of mucoid strains of P. aeruginosa. Therefore, the antibody response to MEP made by the CF patients may be non-opsonic, or if opsonic, may indicate that antibody to this antigen is not protective in CF patients. Antibody to MEP is able to interact with a low level of human complement for opsonic killing, suggesting that this antibody may be of use in the lung of the CF patient, where complement components may be either low or inactive.
铜绿假单胞菌菌株表达的表面多糖抗原易于与宿主免疫系统相互作用。这些相互作用可能会引发针对铜绿假单胞菌感染的保护性免疫。从免疫功能低下宿主和环境中分离出的经典铜绿假单胞菌菌株通常表达含有血清型决定簇的细胞表面脂多糖抗原。针对这些决定簇的抗体已明确显示在动物和人类中均具有保护作用。用从铜绿假单胞菌培养上清液中获得的无毒高分子量多糖组分进行免疫，可诱导产生针对型特异性决定簇的高滴度功能性抗体。此外，动物实验方案表明，针对型特异性抗原的T细胞介导免疫也可提供保护性免疫。尽管T细胞介导免疫在预防铜绿假单胞菌感染中的作用尚不清楚，但它可能在增强抗体介导的保护作用中很重要。来自囊性纤维化（CF）患者的铜绿假单胞菌分离株通常不表达型特异性抗原。然而，它们确实表达一种不同的细胞表面多糖，即MEP。定居的CF宿主会产生针对这种物质的抗体，但显然这与保护性免疫无关。针对这种物质产生的动物抗体能够介导对铜绿假单胞菌黏液型菌株的调理吞噬杀伤作用。因此，CF患者对MEP产生的抗体反应可能是非调理吞噬性的，或者即使是调理吞噬性的，也可能表明针对该抗原的抗体在CF患者中没有保护作用。针对MEP的抗体能够与低水平的人补体相互作用以进行调理吞噬杀伤，这表明这种抗体可能在CF患者的肺部发挥作用，因为肺部的补体成分可能含量低或无活性。
