用正辛基-β-D-吡喃葡萄糖苷提取立氏立克次体的保护成分。

Extraction of protective components of Rickettsia rickettsii with n-octyl-beta-D-glucopyranoside.

作者信息

McDonald G A, Anacker R L, Mann R E

机构信息

Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840.

出版信息

Rev Infect Dis. 1988 Jul-Aug;10 Suppl 2:S382-5. doi: 10.1093/cid/10.supplement_2.s382.

DOI:10.1093/cid/10.supplement_2.s382

PMID:3142019

Abstract

Two Rickettsia rickettsii proteins, with apparent molecular masses of 120 and 155 kilodaltons, have been implicated as important antigens in immunity to Rocky Mountain spotted fever. An n-octyl-beta-D-glucopyranoside-soluble fraction of R. rickettsii was found to be enriched for these proteins along with others. Vaccination of mice with the detergent-soluble fraction protected them against death caused by R. rickettsii and protected guinea pigs from developing Rocky Mountain spotted fever after being challenged with viable R. rickettsii.

摘要

两种立氏立克次体蛋白，表观分子量分别为120和155千道尔顿，被认为是落基山斑疹热免疫中的重要抗原。已发现立氏立克次体的正辛基-β-D-吡喃葡萄糖苷可溶部分富含这些蛋白以及其他蛋白。用去污剂可溶部分对小鼠进行免疫接种可保护它们免于死于立氏立克次体感染，并可保护豚鼠在受到活立氏立克次体攻击后不患落基山斑疹热。