State Key Laboratory of Silicon Materials, School of Materials Science and Engineering, Zhejiang University, Hangzhou, Zhejiang, 310027, PR China.
Key Laboratory of Endoscopic Technique Research of Zhejiang Province, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, 215123, PR China.
Biomaterials. 2019 Nov;221:119419. doi: 10.1016/j.biomaterials.2019.119419. Epub 2019 Aug 9.
An unpredicted side effect of photothermal therapy (PTT) is agitated by hyperthermia which results in damage to healthy tissue. Developing PTT platforms, enabling effective tumor ablation under mild irradiation conditions, is of wide interest, but challenging. Here, we investigated bismuth crystals embedded silica (Bi@SiO) nanoparticles, loaded with an autophagy inhibitor (chloroquine, CQ). It was found that SiO effectively prevented the oxidization of Bi nanocrystals in the physiological environment and could serve as a scatter layer to improve NIR absorption, enabling a high photothermal conversion efficiency (~43%) and excellent photostability. Furthermore, the findings indicated that CQ molecules, delivered intracellularly by the particles, significantly weakened the degradation of autolysosomes by lysosome within the tumor cells, thus inducing suppression effect to autophagy and resistance to photothermia. Both in vitro and in vivo anti-tumor effects were consequently promoted owing to the combined effects enabled by Bi@SiO-CQ nanoparticles under mild NIR irradiation conditions. This study demonstrates a potential new PTT platform with superior therapeutic efficacy.
光热疗法（PTT）的一个意外副作用是由过热引起的，这会导致健康组织受损。开发 PTT 平台，在温和的辐照条件下实现有效的肿瘤消融，引起了广泛的关注，但具有挑战性。在这里，我们研究了嵌入二氧化硅（Bi@SiO）纳米颗粒的铋晶体，负载自噬抑制剂（氯喹，CQ）。研究发现，SiO 能在生理环境中有效阻止 Bi 纳米晶的氧化，并可作为散射层来提高近红外吸收，从而实现高的光热转换效率（约 43%）和优异的光稳定性。此外，研究结果表明，CQ 分子通过颗粒被递送到细胞内，显著减弱了肿瘤细胞内溶酶体对自噬溶酶体的降解，从而诱导自噬抑制和对光热的抗性。由于 Bi@SiO-CQ 纳米颗粒在温和的近红外辐射条件下产生的协同作用，体外和体内的抗肿瘤效果都得到了促进。本研究展示了一种具有优异治疗效果的潜在新型 PTT 平台。
