Key Laboratory of Flexible Electronics (KLOFE) and Institute of Advanced Materials (IAM), School of Physical and Mathematical Sciences, Nanjing Tech University (Nanjing Tech), Nanjing, 211800, China.
School of Physical Science and Information Technology, Liaocheng University, Liaocheng, 252059, China.
Small. 2020 Jun;16(24):e2000436. doi: 10.1002/smll.202000436. Epub 2020 May 13.
The emergence of multidrug resistant bacteria has resulted in plenty of stubborn nosocomial infections and severely threatens human health. Developing novel bactericide and therapeutic strategy is urgently needed. Herein, mesoporous silica supported silver-bismuth nanoparticles (Ag-Bi@SiO NPs) are constructed for synergistic antibacterial therapy. In vitro experiments indicate that the hyperthermia originating from Bi NPs can disrupt cell integrity and accelerate the Ag ions release, further exhibiting an excellent antibacterial performance toward methicillin-resistant Staphylococcus aureus (MRSA). Besides, under laser irradiation, Ag-Bi@SiO NPs at 100 µg mL can effectively obliterate mature MRSA biofilm and cause a 69.5% decrease in the biomass, showing a better therapeutic effect than Bi@SiO NPs with laser (26.8%) or Ag-Bi@SiO NPs without laser treatment (30.8%) groups. More importantly, in vivo results confirm that ≈95.4% of bacteria in abscess are killed and the abscess ablation is accelerated using the Ag-Bi@SiO NPs antibacterial platform. Therefore, Ag-Bi@SiO NPs with photothermal-enhanced antibacterial activity are a potential nano-antibacterial agent for the treatment of skin infections.
多药耐药菌的出现导致了大量顽固的医院感染，并严重威胁着人类健康。因此，迫切需要开发新型杀菌剂和治疗策略。在此，构建了介孔硅负载的银-铋纳米粒子（Ag-Bi@SiO NPs）用于协同抗菌治疗。体外实验表明，Bi NPs 产生的热疗可以破坏细胞完整性并加速 Ag 离子的释放，从而对耐甲氧西林金黄色葡萄球菌（MRSA）表现出优异的抗菌性能。此外，在激光照射下，浓度为 100μg/mL 的 Ag-Bi@SiO NPs 可以有效消除成熟的 MRSA 生物膜，并使生物量减少 69.5%，显示出比单独使用激光的 Bi@SiO NPs（26.8%）或不使用激光处理的 Ag-Bi@SiO NPs（30.8%）更好的治疗效果。更重要的是，体内结果证实，使用 Ag-Bi@SiO NPs 抗菌平台后，脓肿中约 95.4%的细菌被杀死，脓肿消融速度加快。因此，具有光热增强抗菌活性的 Ag-Bi@SiO NPs 是一种有潜力的用于治疗皮肤感染的纳米抗菌剂。
