作为二胺氧化酶（DAO）活性指标的14C-腐胺代谢物总量。柱色谱分析。
A sum of 14C-putrescine metabolites as a measure of DAO activity. Column chromatography assay.
作者信息
Fogel W A, Ulatowska M, Adach K, Osińska Z
出版信息
Agents Actions. 1985 Apr;16(3-4):99-101. doi: 10.1007/BF01983111.
PMID:3925737
Abstract
The rapid ion-exchange chromatographic method was developed for the separation of 14C-putrescine from its metabolites arising from diamine oxidase (DAO, E.C. 1.4.3.6) catalysed reaction. The assay is linear for putrescine conversion with both, time and enzyme concentration. With this method many samples could be analysed simultaneously.
摘要
已开发出一种快速离子交换色谱法，用于从二胺氧化酶（DAO，E.C. 1.4.3.6）催化反应产生的代谢产物中分离14C-腐胺。该测定法对于腐胺转化在时间和酶浓度方面均呈线性。使用这种方法可以同时分析许多样品。
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