Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto, Pisa, Italy.
J Insect Sci. 2019 Jul 1;19(4). doi: 10.1093/jisesa/iez080.
Torymus sinensis Kamijo (Hymenoptera: Torymidae) is an alien parasitoid that is used in many areas of the world for biological control the Asian chestnut gall wasp, Dryocosmus kuriphilus Yasumatsu (Hymenoptera: Cynipidae). In Italy, this parasitoid was imported from Japan in 2003 and subsequently multiplied and released throughout the country. In this study, a phylogenetic investigation was carried out on insects from three different sites in northern Tuscany (Italy). Moreover, the possible hybridization between T. sinensis and some native Torymus species was evaluated. The conserved region 18S rRNA gene and the hypervariable ITS2 (Internal Transcribed Spacer 2) region of the ribosomal cistrone were selected as molecular markers. Sequencing the amplified products, after cloning, ruled out any hybridization between T. sinensis and the native Torymus species, and also confirmed the presence of two haplotypes for the Tuscan population of T. sinensis both for the region of the 18S rRNA gene as well as for the ITS2 region. These results confirm that the environmental impact of the alien parasitoid T. sinensis in the study site is acceptable, although an extensive and repeated monitoring would be desirable.
中华长尾小蜂（膜翅目：长尾小蜂科）是一种外来寄生蜂，被广泛用于世界各地的生物防治亚洲栗瘿蜂（膜翅目：瘿蜂科）。在意大利，这种寄生蜂于 2003 年从日本引进，并在全国范围内繁殖和释放。本研究对来自意大利托斯卡纳北部三个不同地点的昆虫进行了系统发育研究。此外，还评估了中华长尾小蜂与一些本地长尾小蜂种之间可能的杂交情况。选择保守的 18S rRNA 基因和核糖体基因内转录间隔区 2（ITS2）的高变区作为分子标记。扩增产物测序后，克隆排除了中华长尾小蜂与本地长尾小蜂种之间的任何杂交，并证实了托斯卡纳中华长尾小蜂种群的存在，两个单倍型既存在于 18S rRNA 基因区域，也存在于 ITS2 区域。这些结果证实，尽管需要进行广泛和重复的监测，但研究地点外来寄生蜂中华长尾小蜂的环境影响是可以接受的。
