Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310058, China.
Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310058, China.
J Hazard Mater. 2020 Jan 5;381:120995. doi: 10.1016/j.jhazmat.2019.120995. Epub 2019 Aug 12.
Despite the great concerns associated with the combined biological effects of nanoparticles and insecticides, the current understanding of the corresponding ecological risks remains limited. Xenopus laevis (X. laevis) tadpoles were exposed to various concentrations of typical pyrethroid (cis-bifenthrin; cis-BF), either alone or in combination with graphene oxide (GO), for 21 days. The presence of GO resulted in increased bioconcentration of cis-BF and a higher 1S-enantiomer fraction. Exposure to cis-BF and GO caused further reduction in pre-metamorphic developmental rates and activated dopaminergic, noradrenergic, and serotonergic neurotransmitter systems. Reduced tadpole activity and levels of genomic DNA methylation at cytosine nucleotides (5hmC) were observed in the coexposure groups. These results indicate that GO enhance the bioconcentration of cis-BF and promote the conversion of its 1R-enantiomer to the 1S form, which lead to disruption of neurotransmitter systems as well as interference in metamorphic development.
尽管纳米颗粒和杀虫剂联合的生物效应引起了极大关注，但目前对相应的生态风险的认识仍然有限。非洲爪蟾（X. laevis）蝌蚪在 21 天内暴露于不同浓度的典型拟除虫菊酯（顺式-溴氰菊酯；cis-BF）中，要么单独暴露，要么与氧化石墨烯（GO）一起暴露。GO 的存在导致 cis-BF 的生物浓缩增加，并且 1S-对映体分数更高。暴露于 cis-BF 和 GO 会进一步降低前变态发育速度，并激活多巴胺能、去甲肾上腺素能和 5-羟色胺能神经递质系统。在共暴露组中观察到蝌蚪活性降低和胞嘧啶核苷酸（5hmC）的基因组 DNA 甲基化水平降低。这些结果表明，GO 增强了 cis-BF 的生物浓缩作用，并促进了其 1R-对映体向 1S 形式的转化，从而导致神经递质系统的破坏以及变态发育的干扰。
