Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, United States.
Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, United States; Center for Stem Cell and Organoid Medicine (CuSTOM), Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, United States; Division of Endocrinology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, United States.
Curr Opin Cell Biol. 2019 Dec;61:92-100. doi: 10.1016/j.ceb.2019.07.009. Epub 2019 Aug 16.
The utilization of directed differentiation of human pluripotent stem cells to generate human tissues is quickly evolving. Here we review recent advances in the derivation and applications of human endodermal tissues, including the esophagus, lung, pancreas, liver, stomach, small intestine, and colon. Improvements in tissue transcriptional and functional maturation, multicellular complexity, and scalability allow better development and disease modeling, large-scale drug and toxicity screening, and potentially cell therapeutic applications.
人多能干细胞的定向分化在生成人体组织方面发展迅速。本文综述了人内胚层组织（包括食管、肺、胰腺、肝、胃、小肠和结肠）的最新衍生和应用进展。组织转录和功能成熟、细胞间复杂性和可扩展性的提高，使得更好的发育和疾病建模、大规模药物和毒性筛选以及潜在的细胞治疗应用成为可能。
