Hartley D A, Preiss A, Artavanis-Tsakonas S
Department of Biology, Yale University, New Haven, Connecticut 06511.
Cell. 1988 Dec 2;55(5):785-95. doi: 10.1016/0092-8674(88)90134-1.
The correct segregation of neural from epidermal lineages in Drosophila embryogenesis depends on the activity of the six zygotic "neurogenic" genes. One of the neurogenic genes, Enhancer of split, is particularly noteworthy in its genetic interactions with Notch and Delta, which both appear to code for transmembrane proteins with homology to the epidermal growth factor. Transformation experiments have demonstrated the cloning of sequences necessary for Enhancer of split gene function. We report here that the gene product derived from DNA sequencing shows homology to the beta subunit of mammalian G proteins and CDC4, a yeast cell cycle gene. We demonstrate that expression of the transcripts relates to the developing central nervous system. These data suggest a mechanism of interaction between the gene products of Notch and Enhancer of split.
在果蝇胚胎发育过程中，神经谱系与表皮谱系的正确分离取决于六个合子“神经源”基因的活性。其中一个神经源基因“分裂增强子”，在其与Notch和Delta的遗传相互作用方面尤为值得关注，Notch和Delta似乎都编码与表皮生长因子具有同源性的跨膜蛋白。转化实验已证明了分裂增强子基因功能所需序列的克隆。我们在此报告，通过DNA测序得到的基因产物与哺乳动物G蛋白的β亚基以及酵母细胞周期基因CDC4具有同源性。我们证明这些转录本的表达与发育中的中枢神经系统相关。这些数据提示了Notch和分裂增强子基因产物之间的相互作用机制。
