Wharton K A, Johansen K M, Xu T, Artavanis-Tsakonas S
Cell. 1985 Dec;43(3 Pt 2):567-81. doi: 10.1016/0092-8674(85)90229-6.
The primary structure of the major embryonic Notch transcript is presented, as determined by sequence analysis of overlapping cDNA clones. The 10,148 bp sequence corresponding to this transcript possesses an 8109 bp open reading frame that potentially codes for a 2703 amino acid protein. We show that this polypeptide contains a repeated structure composed of 36 tandemly arranged 40 amino acid long repeats, which show homology to the epidermal growth factor and other proteins containing EGF-like repeats. Hydropathy plots suggest that the putative Notch protein may span the membrane. We relate these findings to the developmental action of Notch and speculate that the locus may be involved in a cell-cell interaction mechanism that is essential for the differentiation of the ectoderm into neural and epidermal precursors.
通过对重叠cDNA克隆的序列分析，展示了主要胚胎Notch转录本的一级结构。与该转录本对应的10148 bp序列具有一个8109 bp的开放阅读框，可能编码一个2703个氨基酸的蛋白质。我们发现该多肽包含一个由36个串联排列的40个氨基酸长的重复序列组成的重复结构，这些重复序列与表皮生长因子及其他含有EGF样重复序列的蛋白质具有同源性。亲水性图谱表明，推测的Notch蛋白可能跨膜。我们将这些发现与Notch的发育作用相关联，并推测该基因座可能参与了一种细胞-细胞相互作用机制，这种机制对于外胚层分化为神经和表皮前体至关重要。
