由分泌的细胞外囊泡参与细胞壁重塑。

Extracellular vesicles secreted by are involved in cell wall remodelling.

机构信息

1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086 Australia.

2Department of Computer Science and Information Technology, La Trobe University, Melbourne, VIC 3086 Australia.

出版信息

Commun Biol. 2019 Aug 9;2:305. doi: 10.1038/s42003-019-0538-8. eCollection 2019.

Extracellular vesicles (EVs) are membranous vesicles that are released by cells. In this study, the role of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery in the biogenesis of yeast EVs was examined. Knockout of components of the ESCRT machinery altered the morphology and size of EVs as well as decreased the abundance of EVs. In contrast, strains with deletions in cell wall biosynthesis genes, produced more EVs than wildtype. Proteomic analysis highlighted the depletion of ESCRT components and enrichment of cell wall remodelling enzymes, glucan synthase subunit Fks1 and chitin synthase Chs3, in yeast EVs. Interestingly, EVs containing Fks1 and Chs3 rescued the yeast cells from antifungal molecules. However, EVs from ∆ or ∆ or the ∆∆ double knockout strain were unable to rescue the yeast cells as compared to ∆ EVs. Overall, we have identified a potential role for yeast EVs in cell wall remodelling.

细胞外囊泡（EVs）是由细胞释放的膜性囊泡。在这项研究中，研究了内体分选复合物必需的运输（ESCRT）机制在酵母 EVs 生物发生中的作用。ESCRT 机制成分的敲除改变了 EVs 的形态和大小，并降低了 EVs 的丰度。相比之下，细胞壁生物合成基因缺失的菌株产生的 EVs 比野生型多。蛋白质组学分析突出了 ESCRT 成分的耗竭和细胞壁重塑酶、葡聚糖合成酶亚基 Fks1 和几丁质合成酶 Chs3 的富集，这些酶在酵母 EVs 中。有趣的是，含有 Fks1 和 Chs3 的 EVs 可使酵母细胞免受抗真菌分子的侵害。然而，与 ∆ EVs 相比，来自 ∆ 或 ∆ 或 ∆∆ 双敲除菌株的 EVs 无法挽救酵母细胞。总的来说，我们已经确定了酵母 EVs 在细胞壁重塑中的潜在作用。