Jabalera Ylenia, Fernández-Vivas Antonia, Iglesias Guillermo R, Delgado Ángel V, Jimenez-Lopez Concepcion
Department of Microbiology, Faculty of Sciences, University of Granada, Spain.
Department of Applied Physics, Faculty of Sciences, University of Granada, Spain.
Colloids Surf B Biointerfaces. 2019 Nov 1;183:110435. doi: 10.1016/j.colsurfb.2019.110435. Epub 2019 Aug 8.
Recently, liposomes have been explored as a potential solution to improve the biocompatibility and the colloidal stability of magnetic nanoparticles. Protocols have been developed for producing magnetoliposomes of magnetite nanoparticles obtained inorganically (MNPs). However, the biomimetic synthesis of magnetite using heterologous proteins from magnetotactic bacteria has become a real alternative to produce novel biomimetic magnetic nanoparticles (BMNPs). Among these, the BMNPs obtained in presence of MamC protein from Magnetococcus marinus MC-1 have been proposed as excellent candidates to be potentially used as drug nanocarriers and as hyperthermia agents. However, their colloidal stability still needs to be improved while maintaining their magnetic properties intact. One possibility explored in this manuscript is to form magnetoliposomes that contain BMNPs. Indeed, the protocols developed for producing magnetoliposomes of MNPs need to be tested and modified to be able to include BMNPs. In this context, a protocol has been developed to produce both magnetoliposomes filled with MNPs and/or BMNPs and their potential as hyperthermia agents was tested. In fact, for the first time, these two types of nanoparticles were mixed in different proportions to test the composition that would optimize such as behaviour as hyperthermia agents. Interestingly, it was observed that the hyperthermia behaviour of the magnetoliposomes greatly improved if they were filled with a mixture of MNPs and BMNPs. These results indicate that these magnetoliposomes display optimal characteristics to become a potential agent for hyperthermia and that the opening of those liposomes could be externally controlled by applying an alternate magnetic field.
最近，脂质体已被探索作为一种潜在的解决方案，以提高磁性纳米颗粒的生物相容性和胶体稳定性。已经开发出了生产通过无机方式获得的磁铁矿纳米颗粒（MNPs）的磁脂质体的方案。然而，利用趋磁细菌的异源蛋白进行磁铁矿的仿生合成已成为生产新型仿生磁性纳米颗粒（BMNPs）的一种切实可行的替代方法。其中，在来自海磁球菌MC-1的MamC蛋白存在下获得的BMNPs被认为是有潜力用作药物纳米载体和热疗剂的优秀候选物。然而，它们的胶体稳定性在保持其磁性完整的同时仍有待提高。本手稿中探索的一种可能性是形成含有BMNPs的磁脂质体。确实，为生产MNPs的磁脂质体而开发的方案需要进行测试和修改，以便能够纳入BMNPs。在此背景下，已开发出一种方案来生产填充有MNPs和/或BMNPs的磁脂质体，并测试了它们作为热疗剂的潜力。事实上，首次将这两种类型的纳米颗粒以不同比例混合，以测试能优化其作为热疗剂性能的组成。有趣的是，观察到如果磁脂质体填充有MNPs和BMNPs的混合物，其热疗性能会大大提高。这些结果表明，这些磁脂质体展现出成为潜在热疗剂的最佳特性，并且通过施加交变磁场可以从外部控制这些脂质体的打开。
