Department of Microbiology, University of Granada, Faculty of Sciences, Campus de Fuentenueva s/n, 18002 Granada, Spain.
Freie Universität Berlin, Institute of Chemistry and Biochemistry, Chemistry, Takustr. 3, 14195 Berlin, Germany.
J Mater Chem B. 2020 Jun 10;8(22):4870-4882. doi: 10.1039/c9tb02480h.
Biomimetic magnetite nanoparticles (BMNPs) synthesized in the presence of MamC, a magnetosome-associated protein from Magnetoccus marinus MC-1, have gained interest for biomedical applications because of their unique magnetic properties. However, their behavior in biological systems, like their interaction with proteins, still has to be evaluated prior to their use in clinics. In this study, doxorubicin (DOXO) as a model drug was adsorbed onto BMNPs to form nanoassemblies. These were incubated with human plasma to trigger protein corona (PC) formation. Proteins from the human plasma stably attached to either BMNPs or DOXO-BMNP nanoassemblies. In particular, fibrinogen was detected as the main component in the PC of DOXO-BMNPs that potentially provides advantages, e.g. protecting the particles from phagocytosis, thus prolonging their circulation time. Adsorption of PC to the BMNPs did not alter their magnetic properties but improved their colloidal stability, thus reducing their toxicity in human macrophages. In addition, PC formation enhanced cellular internalization and did not interfere with DOXO activity. Overall, our data indicate that the adsorption of PC onto DOXO-BMNPs in biological environment even increases their efficiency as drug carrier systems.
仿生磁铁矿纳米颗粒（BMNPs）在玛氏球菌 MC-1 的磁小体相关蛋白 MamC 的存在下合成，由于其独特的磁性而引起了生物医学应用的关注。然而，在将其用于临床之前，仍需要评估它们在生物系统中的行为，例如它们与蛋白质的相互作用。在这项研究中，阿霉素（DOXO）作为模型药物被吸附到 BMNPs 上形成纳米组装体。将这些纳米组装体与人体血浆孵育以触发蛋白质冠（PC）形成。来自人体血浆的蛋白质稳定地附着在 BMNPs 或 DOXO-BMNP 纳米组装体上。特别是，纤维蛋白原被检测为 DOXO-BMNPs 中 PC 的主要成分，这可能提供了一些优势，例如保护颗粒免受吞噬作用，从而延长其循环时间。PC 的吸附不会改变 BMNPs 的磁性，但会提高其胶体稳定性，从而降低其在人巨噬细胞中的毒性。此外，PC 的形成增强了细胞内化，并且不干扰 DOXO 的活性。总体而言，我们的数据表明，在生物环境中 DOXO-BMNPs 上 PC 的吸附甚至会提高其作为药物载体系统的效率。
