Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Int J Syst Evol Microbiol. 2019 Nov;69(11):3603-3608. doi: 10.1099/ijsem.0.003669.
A novel actinomycete, designated strain GLM-1, was isolated from arbuscular mycorrhizal fungal spores from RYA08, collected from Pierre ex Lec. rhizosphere soil in Klaeng, Rayong Province, Thailand. Morphological characteristics of this strain included long chains of rod-like cells and squarish elements. The cell-wall composition of this novel isolate contained -diaminopimelic acid. The whole-cell diagnostic sugars were arabinose and galactose. The predominant menaquinone was MK-9(H). The major fatty acids were iso-C and iso-C. Only phosphatidylethanolamine was detected as a polar lipid. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GLM-1 was closely related to SB026 (99.11 %) with a low DNA-DNA hybridization value of 22.6-34.7 %. Genome sequencing revealed a genome size of 10 Mbp. There were obvious distinctions in the average nucleotide identity values between stain GLM-1 and its closely related strains at around 86-93 % (ANIb) and 89-94 % (ANIm). The digital DNA-DNA hybridization values between strain GLM-1 and type strains of phylogenetically related species were 34-55 %. The G+C content of the genomic DNA was 71.8 mol%. Based on these data, strain GLM-1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GLM-1 (=TBRC 9315=NBRC 113658).
一株新的放线菌，命名为 GLM-1 菌株，是从泰国 Rayong 省 Klaeng 的 Pierre ex Lec. 根际土壤中收集的丛枝菌根真菌孢子中分离得到的。该菌株的形态特征包括长链的杆状细胞和方形元件。该新分离株的细胞壁组成含有二氨基庚二酸。全细胞诊断糖是阿拉伯糖和半乳糖。主要的类异戊二烯醌是 MK-9(H)。主要脂肪酸是 iso-C 和 iso-C。只有磷脂酰乙醇胺被检测为极性脂质。基于 16S rRNA 基因序列的系统发育分析表明，GLM-1 菌株与 SB026（99.11%）密切相关，DNA-DNA 杂交值为 22.6-34.7%。基因组测序显示基因组大小为 10 Mbp。GLM-1 菌株与其密切相关菌株之间的平均核苷酸同一性值存在明显差异，约为 86-93%（ANIb）和 89-94%（ANIm）。GLM-1 菌株与系统发育相关种的模式菌株之间的数字 DNA-DNA 杂交值为 34-55%。基因组 DNA 的 G+C 含量为 71.8 mol%。基于这些数据，GLM-1 菌株被认为代表了一个新的属种，建议将其命名为 sp. nov.。其模式菌株为 GLM-1（=TBRC 9315=NBRC 113658）。
