Helmholtz Centre for Infection Research (HZI), Microbial Strain Collection (MISG), 38124, Brunswick, Germany.
Research Center for Environmental and Clean Technology, National Research and Innovation Agency (BRIN), Bandung, 40135, Indonesia.
Arch Microbiol. 2022 Jul 14;204(8):488. doi: 10.1007/s00203-022-03099-y.
Three new bacterial strains, WHY3, WH131, and WH158, were isolated and described from the hemolymph of the Pacific oyster Crassostrea gigas utilizing polyphasic taxonomic techniques. The 16S rRNA gene sequence analysis revealed that strain WHY3 was a member of the genus Winogradskyella, whereas strains WHI31 and WH158 were members of the genus Erythrobacter. According to the polygenomic study the three strains formed individual lineages with strong bootstrap support. The comparison of dDDH-and ANI values, percentage of conserved proteins (POCP), and average amino acid identity (AAl) between the three strains and their relatives established that the three strains represented two separate genera. Menaquinone-6 was reported as the major respiratory quinone in strain WHY3 and Ubiquinone-10 for strains WH131 and WH158, respectively. The major cellular fatty acids for strain WHY3 were C, anteiso-C ω7c, iso-C, Cω7c. The major cellular fatty acids for strains WH131 and WH158 were C2-OH and tω12 for WH131 and C and Cω7c for strain WH158. Positive Sudan Black B staining Indicated the presence of polyhydroxyalkanoic acid granules for strains WH131 and WH158 but not for strain WHY3. The DNA G + C contents of strains WHY3, WH131 and WH158 were 34.4, 59.7 and 56.6%, respectively. Gene clusters predicted some important genes involved in the bioremediation process. Due to the accomplishment of polyphasic taxonomy, we propose three novel species Winogradskyella luteola sp.nov. (type strain WHY3 = DSM 111804 = NCCB 100833), Erythrobacter ani sp.nov. (WH131 = DSM 112099 = NCCB 100824) and Erythrobacter crassostrea sp.nov. (WH158 = DSM 112102 = NCCB 100877).
利用多相分类技术，从太平洋牡蛎（Crassostrea gigas）的血淋巴中分离并描述了三个新的细菌菌株，分别为 WHY3、WH131 和 WH158。16S rRNA 基因序列分析表明，菌株 WHY3 是 Winogradskyella 属的成员，而菌株 WHI31 和 WH158 是 Erythrobacter 属的成员。根据多基因组研究，这三个菌株形成了具有强烈自举支持的单独谱系。三个菌株与其亲缘关系的 dDDH 和 ANI 值、保守蛋白百分比 (POCP) 和平均氨基酸同一性 (AAI) 的比较表明，三个菌株代表两个不同的属。menaquinone-6 被报道为菌株 WHY3 的主要呼吸醌，而 ubiquinone-10 则是菌株 WH131 和 WH158 的主要呼吸醌。菌株 WHY3 的主要细胞脂肪酸为 C、anteiso-C ω7c、iso-C、Cω7c。菌株 WH131 和 WH158 的主要细胞脂肪酸为 C2-OH 和 tω12，WH131 的主要细胞脂肪酸为 C 和 Cω7c。苏丹黑 B 染色阳性表明菌株 WH131 和 WH158 中存在聚羟基烷酸颗粒，但菌株 WHY3 中不存在。菌株 WHY3、WH131 和 WH158 的 DNA G+C 含量分别为 34.4%、59.7%和 56.6%。基因簇预测了一些参与生物修复过程的重要基因。由于完成了多相分类学，我们提出了三个新的物种，分别是 Winogradskyella luteola sp. nov.（模式菌株 WHY3=DSM 111804=NCCB 100833）、Erythrobacter ani sp. nov.（WH131=DSM 112099=NCCB 100824）和 Erythrobacter crassostrea sp. nov.（WH158=DSM 112102=NCCB 100877）。
