Penkhrue Watsana, Sujarit Kanaporn, Kudo Takuji, Ohkuma Moriya, Masaki Kazuo, Aizawa Tomoyasu, Pathom-Aree Wasu, Khanongnuch Chartchai, Lumyong Saisamorn
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Graduate School, Chiang Mai University, Chiang Mai 50200, Thailand.
Int J Syst Evol Microbiol. 2018 May;68(5):1448-1454. doi: 10.1099/ijsem.0.002682. Epub 2018 Mar 8.
A novel bioplastic-degrading actinomycete, strain SCM_MK2-4, was isolated from paddy soil in Thailand. The 16S rRNA gene sequence showed that strain SCM_MK2-4 belonged to the genus Amycolatopsis, with the highest sequence similarity to Amycolatopsisazurea JCM 3275 (99.4 %), and was phylogenetically clustered with this strain along with Amycolatopsislurida JCM 3141 (99.3 %), A. japonica DSM 44213 (99.2 %), A. decaplanina DSM 44594 (99.0 %), A. roodepoortensis M29 (98.9 %), A. keratiniphilasubsp. nogabecina DSM 44586 (98.8 %), A. keratiniphilasubsp. keratiniphila DSM 44409 (98.5 %), A. orientalis DSM 40040 (98.4 %) and A. regifaucium GY080 (98.3 %). A combination of DNA-DNA hybridization results ranging from 42.8±3.2 to 66.2±1.4 % with the type strains of A. azurea and A. lurida and some different phenotypic characteristics indicated that the strain could be distinguished from its closest phylogenetic neighbours. Whole-cell hydrolysates of the strain were shown to contain meso-diaminopimelic acid, arabinose, galactose, glucose, ribose, mannose, rhamnose and xylose. The predominant menaquinone was MK-9(H4). The major cellular fatty acid profile consisted of iso-C15 : 0, iso-C16 : 0, summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2OH) and C16 : 0. The polar lipid composition of the strain consisted of phosphatidyl-N-methylethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, aminophospholipids, an unidentified phospholipid and two unidentified glycolipids. The G+C content of the genomic DNA was 68.2 mol%. On the basis of phylogenetic analyses, DNA-DNA hybridization experimentation and the phenotypic characteristics, it was concluded that strain SCM_MK2-4 represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis oliviviridis sp. nov. is proposed. The type strain is SCM_MK2-4 (=TBRC 7186=JCM 32134).
从泰国稻田土壤中分离出一株新型的可降解生物塑料的放线菌，菌株SCM_MK2 - 4。16S rRNA基因序列显示，菌株SCM_MK2 - 4属于拟无枝酸菌属，与蔚蓝拟无枝酸菌JCM 3275的序列相似性最高（99.4%），在系统发育上与该菌株以及鲁氏拟无枝酸菌JCM 3141（99.3%）、日本拟无枝酸菌DSM 44213（99.2%）、脱铂拟无枝酸菌DSM 44594（99.0%）、鲁德波特拟无枝酸菌M29（98.9%）、嗜角蛋白拟无枝酸菌亚种nogabecina DSM 44586（98.8%）、嗜角蛋白拟无枝酸菌亚种嗜角蛋白拟无枝酸菌DSM 44409（98.5%）、东方拟无枝酸菌DSM 40040（98.4%）和雷吉法拟无枝酸菌GY080（98.3%）聚类在一起。与蔚蓝拟无枝酸菌和鲁氏拟无枝酸菌的模式菌株的DNA - DNA杂交结果在42.8±3.2%至66.2±1.4%之间，以及一些不同的表型特征表明，该菌株可与其最相近的系统发育邻居区分开来。该菌株的全细胞水解产物显示含有内消旋二氨基庚二酸、阿拉伯糖、半乳糖、葡萄糖、核糖、甘露糖、鼠李糖和木糖。主要的甲基萘醌是MK - 9（H4）。主要的细胞脂肪酸谱由异C15 : 0、异C16 : 0、总和特征3（C16 : 1ω7c和/或异C15 : 0 2OH）和C16 : 0组成。该菌株的极性脂质组成包括磷脂酰 - N - 甲基乙基乙醇胺、磷脂酰乙醇胺、羟基磷脂酰乙醇胺、磷脂酰甘油、氨基磷脂、一种未鉴定的磷脂和两种未鉴定的糖脂。基因组DNA的G + C含量为68.2 mol%。基于系统发育分析、DNA - DNA杂交实验和表型特征，得出结论：菌株SCM_MK2 - 4代表拟无枝酸菌属的一个新物种，为此提出新名称橄榄绿拟无枝酸菌（Amycolatopsis oliviviridis sp. nov.）。模式菌株为SCM_MK2 - 4（=TBRC 7186 =JCM 32134）。
