Department of Food Science and Biotechnology, Gachon University, Seongnam, 461-701, Republic of Korea.
Department of Horticultural Life Science, Hankyong National University, Anseong-si, Gyeonggi-do, 17579, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Nov;70(11):5841-5847. doi: 10.1099/ijsem.0.004486. Epub 2020 Sep 23.
A Gram-stain-negative, aerobic, non-motile and rod- or coccoid-shaped novel bacterial strain, designated MAH-25, was isolated from soil sampled in a pine garden. The colonies were observed to be light pink-coloured, smooth, spherical and 1-2 mm in diameter when grown on nutrient agar for 2 days. Strain MAH-25 was found to be able to grow at 15-35 °C, at pH 5.0-8.0 and at 0-2.0 % NaCl. Cell growth occurred on Reasoner's 2A agar and nutrient agar. The strain was found to be positive in both oxidase and catalase tests. According to 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus and closely related to 5-10 (98.0 % similarity), TMB834 (97.7 %), TTB310 (97.6 %) and YS3.2.7 (97.3 %). The average nucleotide identity and digital DNA-DNA hybridization values between strain MAH-25 and the four closely related type strains were in the range of 78.8-81.3 % and 22.3-24.1 %, respectively. The novel strain MAH-25 has a draft genome size of 5 505 957 bp (11 contigs), annotated with 5210 protein-coding genes, 46 tRNA and three rRNA genes. The genomic DNA G+C content was determined to be 70.3 mol%. The predominant isoprenoid quinone was ubiquinone 8 (Q-8). The major fatty acids were identified as C, summed feature 3 (C 7 and/or C 6) and summed feature 8 (C 7 and/or C 6). The main polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. On the basis of DNA-DNA hybridization, genotypic analysis, chemotaxonomic and physiological data, strain MAH-25 represents a novel species within the genus , for which the name sp. nov. is proposed, with MAH-25 (=KACC 19839=CGMCC1.13660) as the type strain.
一株革兰氏阴性、需氧、不运动、杆状或球状的新型细菌菌株，被命名为 MAH-25，从松林花园的土壤中分离出来。当在营养琼脂上培养 2 天时，观察到的菌落呈浅粉色、光滑、球形，直径为 1-2mm。发现菌株 MAH-25 能够在 15-35°C、pH5.0-8.0 和 0-2.0%NaCl 下生长。细胞生长发生在 Reasoner's 2A 琼脂和营养琼脂上。该菌株在氧化酶和过氧化氢酶试验中均呈阳性。根据 16S rRNA 基因序列比较，该分离株被鉴定为属的一个成员，与 5-10（98.0%相似性）、TMB834（97.7%）、TTB310（97.6%）和 YS3.2.7（97.3%）最为密切相关。菌株 MAH-25 与 4 个密切相关的模式菌株之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别在 78.8-81.3%和 22.3-24.1%之间。新型菌株 MAH-25 的基因组大小为 5505957bp（11 个 contigs），注释了 5210 个蛋白质编码基因、46 个 tRNA 和 3 个 rRNA 基因。基因组 DNA G+C 含量为 70.3%mol。主要的类异戊二烯醌是泛醌 8（Q-8）。主要脂肪酸鉴定为 C、总和特征 3（C7 和/或 C6）和总和特征 8（C7 和/或 C6）。主要极性脂是磷脂酰乙醇胺、磷脂酰甘油和双磷脂酰甘油。基于 DNA-DNA 杂交、基因型分析、化学分类和生理数据，菌株 MAH-25 代表属内的一个新型物种，提议使用 sp. nov. 命名法，MAH-25（=KACC 19839=CGMCC1.13660）作为模式株。
