Department of Medical Devices, National Institute of Pharmaceutical Education and Research (NIPER)-Ahmedabad, Ahmedabad, India.
J Biomed Mater Res A. 2019 Dec;107(12):2835-2847. doi: 10.1002/jbm.a.36785. Epub 2019 Aug 28.
Poly(methyl methacrylate) (PMMA) bone cement is the most widely used grouting material in the joint arthroplasties and vertebroplasties. The present investigation has been carried out to scavenge the radicals and monomer by addition of an antioxidant to minimize the toxicity of bone cement (BC). The in silico studies were employed to determine the potent natural antioxidant at physiological conditions. The antioxidant methionine demonstrated a strong binding affinity with free radicals and methyl methacrylate (MMA) monomer than cysteine. The designated amount of methionine was optimized by various assay methods and >2% methionine shows strong scavenging capacity in BC. Moreover, the antioxidant-loaded BC (ABC) demonstrated similar handling, physicochemical and mechanical properties to pristine bone cement. Significantly, the developed formulation shows superior biological characteristics such as cell proliferation (2 ± 1 BC and 6 ± 1 ABC), adhesion (0.32 ± 0.02 BC and 0.54 ± 0.01 ABC), and cell viability (81 ± 2% BC and 93 ± 1% ABC) toward human osteoblast-like cells (MG-63). Therefore, the novel antioxidant bone cement is a potential candidate for various orthopedic applications to eliminate the adverse effects, related to residual toxic radical and monomer in bone cement.
聚甲基丙烯酸甲酯（PMMA）骨水泥是关节置换和椎体成形术中最广泛使用的灌浆材料。本研究通过添加抗氧化剂来清除自由基和单体，以最大限度地降低骨水泥（BC）的毒性。采用计算机模拟研究来确定在生理条件下具有强大抗氧化能力的天然物质。结果表明，蛋氨酸与自由基和甲基丙烯酸甲酯（MMA）单体的结合亲和力强于半胱氨酸。通过各种测定方法对指定量的蛋氨酸进行了优化，结果表明，>2%的蛋氨酸在 BC 中具有很强的清除能力。此外，负载抗氧化剂的 BC（ABC）表现出与原始骨水泥相似的处理、物理化学和机械性能。重要的是，与传统骨水泥相比，所开发的配方具有更好的生物学特性，如细胞增殖（2 ± 1 BC 和 6 ± 1 ABC）、黏附性（0.32 ± 0.02 BC 和 0.54 ± 0.01 ABC）和细胞活力（81 ± 2% BC 和 93 ± 1% ABC），对人成骨样细胞（MG-63）具有更高的生物活性。因此，新型抗氧化骨水泥是各种骨科应用的潜在候选材料，可消除骨水泥中残留的有毒自由基和单体的不良影响。
