Li Xiujuan, Gao Tinghuan, Wang Shoucheng, Zhang Qing, Chen Siyu, Zhang Hongshuo, Xu Jinbin, Wang Xinchao, Liu Qianqian
Heze Univeristy, Heze, Shangdong, 274015, China.
Heze Vocational College, Heze, Shangdong, 274015, China.
J Fluoresc. 2024 Nov 5. doi: 10.1007/s10895-024-04018-8.
A fluorescence probe (Probe-ITR) was designed and synthesized for the rapid detection of Zn based on the excited state intramolecular proton transfer (ESIPT) mechanism. The specific recognition ability of Probe-ITR to Zn was tested using UV-VIS and fluorescence emission spectroscopy. The results demonstrated a rapid response within 40s upon addition of an appropriate amount of Zn into the mixed solution of the target probe molecules (DMSO/PBS = 5/5). Under 365 nm ultraviolet light irradiation, the colorless solution changed to yellow-green fluorescence with a 150-folds increase in intensity. Furthermore, the detection limit for specific recognition of Zn by the probe molecule is only 17.3 nmol/L, indicating high sensitivity. The practical application potential of the probe molecules was enhanced by employing on information storage and conducting cell imaging experiments.
基于激发态分子内质子转移（ESIPT）机制设计并合成了一种用于快速检测锌的荧光探针（Probe-ITR）。使用紫外可见光谱和荧光发射光谱测试了Probe-ITR对锌的特异性识别能力。结果表明，在目标探针分子的混合溶液（DMSO/PBS = 5/5）中加入适量锌后，40秒内即可快速响应。在365 nm紫外光照射下，无色溶液变为黄绿色荧光，强度增加了150倍。此外，探针分子对锌特异性识别的检测限仅为17.3 nmol/L，表明灵敏度高。通过进行信息存储和细胞成像实验，增强了探针分子的实际应用潜力。
