Senogles D R, Paul P S, Johnson D W, Muscoplat C C
Clin Exp Immunol. 1979 May;36(2):299-303.
The ontogeny of lymphocyte subpopulations and monocytes was studied in developing bovine foetuses. Fifty-four bovine foetuses ranging in age from 3 to 9 months of gestation were collected and examined for the presence of thymus-derived (T lymphocytes), bone marrow-derived (B lymphocytes) and phagocytic (macrophage) cells from thymus, spleen and peripheral blood. Detection of the cells expressing T lymphocyte surface markers was done using a modified sheep red blood cell rosette assay. Lymphocytes bearing surface immunoglobulin (sIg) were detected by staining with a fluoresceinated rabbit antibody prepared against bovine IgG. The procedure used for detecting monocytes was a latex bead phagocytic assay. In the thymus, the percentage of T lymphocytes was relatively constant throughout gestation at approximately 60-70%, with B lymphocytes remaining at about 1%, whereas the phagocytic cells showed an increase from 1% at 3 months to approximately 8% at term. The spleen showed an increasing percentage of T lymphocytes, from 11% at 3 months to over 40% at term, whereas the percentage of B lymphocytes remained consistent at about 2-3% from 3 months until term. The percentage of monocytes increases from 1% at 3 months to over 20% at 9 months. Peripheral blood T lymphocytes increased steadily from approximately 1% at 3 months to 45% at term. B lymphocyte levels remained low, about 1%, throughout gestation, whereas monocytes increased from 1% at 3 months to 5% at 9 months.
在发育中的牛胎儿中研究了淋巴细胞亚群和单核细胞的个体发生。收集了54头妊娠3至9个月的牛胎儿，检查胸腺、脾脏和外周血中胸腺来源的（T淋巴细胞）、骨髓来源的（B淋巴细胞）和吞噬细胞（巨噬细胞）的存在情况。使用改良的绵羊红细胞玫瑰花结试验检测表达T淋巴细胞表面标志物的细胞。通过用针对牛IgG制备的荧光素化兔抗体染色来检测带有表面免疫球蛋白（sIg）的淋巴细胞。检测单核细胞的方法是乳胶珠吞噬试验。在胸腺中，T淋巴细胞的百分比在整个妊娠期相对恒定，约为60 - 70%，B淋巴细胞保持在约1%，而吞噬细胞从3个月时的1%增加到足月时的约8%。脾脏中T淋巴细胞的百分比增加，从3个月时的11%增加到足月时的超过40%，而B淋巴细胞的百分比从3个月到足月一直保持在约2 - 3%。单核细胞的百分比从3个月时的1%增加到9个月时的超过20%。外周血T淋巴细胞从3个月时的约1%稳步增加到足月时的45%。B淋巴细胞水平在整个妊娠期保持较低，约为1%，而单核细胞从3个月时的1%增加到9个月时的5%。
