实验室评估稳定同位素标记致倦库蚊（双翅目：蠓科）成虫扩散研究。

Laboratory evaluation of stable isotope labeling of Culicoides (Diptera: Ceratopogonidae) for adult dispersal studies.

机构信息

Entomology Branch, Walter Reed Army Institute of Research, Silver Spring, MD, 20910, USA.

Department of Entomology, University of California-Riverside, Riverside, CA, 92521, USA.

出版信息

Parasit Vectors. 2019 Aug 22;12(1):411. doi: 10.1186/s13071-019-3671-9.

DOI:10.1186/s13071-019-3671-9

PMID:31439006

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6704667/

Abstract

BACKGROUND

Stable isotope labeling is a promising method for use in insect mark-capture and dispersal studies. Culicoides biting midges, which transmit several important animal pathogens, including bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV), are small flies that develop in various semi-aquatic habitats. Previous Culicoides dispersal studies have suffered from the limitations of other labeling techniques, and an inability to definitively connect collected adult midges to specific immature development sites.

RESULTS

Adult C. sonorensis were successfully labeled with C and N stable isotopes as larvae developing in a semi-aquatic mud substrate in the laboratory. High and low-dose isotope treatments for both elements significantly enriched midges above the background isotope levels of unenriched controls. Enrichment had no effect on C. sonorensis survival, though a slight (~ 5 day) delay in emergence was observed, and there was no significant effect of pool size on C or N enrichment levels.

CONCLUSIONS

Stable isotope labeling is life-long, and does not interfere with natural insect behaviors. Stable isotope enrichment using C or N shows promise for Culicoides dispersal studies in the field. This method can be used to identify adult dispersal from larval source habitat where a midge developed. It may be possible to detect a single enriched midge in a pool of unenriched individuals, though further testing is needed to confirm the sensitivity of this method.

摘要

背景

稳定同位素标记是一种很有前途的方法，可用于昆虫标记捕获和扩散研究。传播几种重要动物病原体的致倦库蚊（Culicoides biting midges），包括蓝舌病病毒（BTV）和马传贫病毒（EHDV），是一种在各种半水生栖息地发育的小飞虫。以前的致倦库蚊扩散研究受到其他标记技术的限制，无法将收集到的成年致倦库蚊与特定的不成熟发育地点明确联系起来。

结果

成功地将 C 和 N 稳定同位素标记在实验室中发育的半水生泥基质中的幼虫期 C. sonorensis 上。两种元素的高剂量和低剂量同位素处理均使中肠显著高于未富集对照的背景同位素水平。富集对 C. sonorensis 的存活率没有影响，尽管观察到出现时间略有延迟（约 5 天），但种群大小对 C 或 N 的富集水平没有显著影响。